Anticorps Polyclonal de lapin anti-HERC4

HERC4 Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

Avis (1)

N° de cat : 13691-1-AP

Synonymes

hect domain and RLD 4, HERC4, KIAA1593

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Galerie de données de validation

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  WB analysis using 13691-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13691-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13691-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13691-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 13691-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 13691-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 13691-1-AP

  IP Result of anti-HERC4 (IP:13691-1-AP, 4ug; Detection:13691-1-AP 1:800) with HeLa cells lysate 2000ug.

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  IF Staining of HeLa using 13691-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using HERC4 antibody (13691-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu cérébral humain, tissu placentaire humain, tissu testiculaire de rat, tissu testiculaire de souris, tissu testiculaire humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

13691-1-AP cible HERC4 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HERC4 Protéine recombinante Ag4566
• Nom complet: hect domain and RLD 4
• Masse moléculaire calculée: 1049 aa, 118 kDa
• Poids moléculaire observé: 107 kDa
• Numéro d’acquisition GenBank: BC039600
• Symbole du gène: HERC4
• Identification du gène (NCBI): 26091
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IHC,IF,IP	Elife; SOX4 facilitates PGR protein stability and FOXO1 expression conducive for human endometrial decidualization.; Authors - Pinxiu Huang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Chun (Verified Customer) (07-03-2019)

This antibody is excellent.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2,000
• Cell Tissue Type: HEK293 and HaCaT cells