Anticorps Polyclonal de lapin anti-HERC4

HERC4 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13691-1-PBS

Synonymes

Probable E3 ubiquitin-protein ligase HERC4, KIAA1593, HECT-type E3 ubiquitin transferase HERC4, hect domain and RLD 4, HECT domain and RCC1-like domain-containing protein 4

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Galerie de données de validation

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  WB analysis of human brain using 13691-1-AP (same clone as 13691-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13691-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 13691-1-AP (same clone as 13691-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13691-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 13691-1-AP (same clone as 13691-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13691-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 13691-1-AP (same clone as 13691-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13691-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 13691-1-AP (same clone as 13691-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13691-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 13691-1-AP (same clone as 13691-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13691-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 13691-1-AP (same clone as 13691-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13691-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 13691-1-AP (same clone as 13691-1-PBS)

  IP result of anti-HERC4 (IP:13691-1-AP, 4ug; Detection:13691-1-AP 1:800) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 13691-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 13691-1-AP (same clone as 13691-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using HERC4 antibody (13691-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13691-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13691-1-PBS cible HERC4 dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HERC4 Protéine recombinante Ag4566
• Nom complet: hect domain and RLD 4
• Masse moléculaire calculée: 1049 aa, 118 kDa
• Poids moléculaire observé: 107 kDa
• Numéro d’acquisition GenBank: BC039600
• Symbole du gène: HERC4
• Identification du gène (NCBI): 26091
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HERC4, also known as HECT and RLD domain containing E3 ubiquitin protein ligase 4, is a protein encoded by the HERC4 gene in humans. HERC4 is a novel member of HERC family. The mRNA of HERC4 has been found in all examined tissues, with its levels being significantly higher in the brain and testis than in the placenta and heart. (PMID: 28430527)

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