Anticorps Recombinant de lapin anti-HERPUD1

HERPUD1 Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

243054E4

N° de cat : 85627-1-RR

Synonymes

HERP, Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein, KIAA0025, Methyl methanesulfonate, MIF1

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Galerie de données de validation

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  WB analysis of BxPC-3 using 85627-1-RR

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 85627-1-RR (HERPUD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 85627-1-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 85627-1-RR (HERPUD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 85627-1-RR

  LNCaP cells were subjected to SDS PAGE followed by western blot with 85627-1-RR (HERPUD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 85627-1-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 85627-1-RR (HERPUD1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 85627-1-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 85627-1-RR (HERPUD1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules BxPC-3, cellules HeLa
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85627-1-RR cible HERPUD1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HERPUD1 Protéine recombinante Ag34891
• Nom complet: homocysteine-inducible, endoplasmic reticulum stress-inducible, ubiquitin-like domain member 1
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC008320
• Symbole du gène: HERPUD1
• Identification du gène (NCBI): 9709
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein (HERPUD1), also called HERP or MIF1, is component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. HERP is strongly induced by ER stress but rapidly degraded by proteasome. HERP interacts with members of the ubiquilin family, which function as a shuttle factor to deliver ubiquitinated substrates to the proteasome for degradation. HERP is present in activated microglia in senile plaques in the brain of patients with Alzheimer disease, and there is evidence suggesting HERP increase nigro-striatal dopaminergic activity and is able to improve symptoms in patients with Parkinson's disease, levodopa-related dyskinesias and Tardive dyskinesia. MW of HERP is 54 kDa (PMID: 23620059).

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