Anticorps Polyclonal de lapin anti-HES5

HES5 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(15)

N° de cat : 22666-1-AP

Synonymes

bHLHb38, Hairy and enhancer of split 5, HES5, Transcription factor HES 5

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Galerie de données de validation

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  WB analysis using 22666-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22666-1-AP (HES5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 22666-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 22666-1-AP (HES5 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IHC staining of mouse brain using 22666-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22666-1-AP (HES5 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 22666-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22666-1-AP (HES5 Antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IHC: tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 14 publications below
• IHC: See 1 publications below

Informations sur le produit

22666-1-AP cible HES5 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris, vison
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HES5 Protéine recombinante Ag18270
• Nom complet: hairy and enhancer of split 5 (Drosophila)
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 41 kDa
• Numéro d’acquisition GenBank: BC087840
• Symbole du gène: HES5
• Identification du gène (NCBI): 388585
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HES5, also known as bHLHb38, has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG). HES5 is expressed in fetal heart and brain tumors. HES5 as a transcriptional repressor of genes that require a bHLH protein for their transcription and plays an important role as neurogenesis negative regulator. HES5 is detected with molecular weight of 41 kDa according to the publication (PMID: 24286475).

Publications

Species	Application	Title
mouse	WB	Dev Cell; Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination; Authors - Li Li
mouse	WB	Dev Cell; Regulation of reverse electron transfer at mitochondrial complex I by unconventional Notch action in cancer stem cells.; Authors - Rani Ojha
human	WB	Cell Rep; Stub1 maintains proteostasis of master transcription factors in embryonic stem cells.; Authors - Md Mahfuz Al Mamun
human,mouse	WB,IHC	Am J Cancer Res; PRDM16 from hepatic stellate cells-derived extracellular vesicles promotes hepatocellular carcinoma progression; Authors - Chen Sun
rat	WB	Front Pharmacol; Danzhi Xiaoyao Powder Promotes Neuronal Regeneration by Downregulating Notch Signaling Pathway in the Treatment of Generalized Anxiety Disorder.; Authors - Chao Liu
mink	WB	Life Sci; Pantothenic acid promotes dermal papilla cell proliferation in hair follicles of American minks via inhibitor of DNA Binding 3/Notch signaling pathway.; Authors - Zhuo Wang

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