Anticorps Monoclonal anti-HEXA

HEXA Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, Lapin, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3A4F2

N° de cat : 60337-1-PBS

Synonymes

HEXA, Hexosaminidase subunit A, TSD

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Galerie de données de validation

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  WB analysis using 60337-1-Ig (same clone as 60337-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60337-1-Ig (HEXA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60337-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit testis using 60337-1-Ig (same clone as 60337-1-PBS)

  rabbit testis tissue were subjected to SDS PAGE followed by western blot with 60337-1-Ig (HEXA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60337-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 60337-1-Ig (same clone as 60337-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 60337-1-Ig (HEXA Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60337-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60337-1-PBS cible HEXA dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, rat, souris

• Réactivité: Humain, Lapin, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HEXA Protéine recombinante Ag1854
• Nom complet: hexosaminidase A (alpha polypeptide)
• Masse moléculaire calculée: 529 aa, 60 kDa
• Poids moléculaire observé: 50-54 kDa, 60-67 kDa
• Numéro d’acquisition GenBank: BC018927
• Symbole du gène: HEXA
• Identification du gène (NCBI): 3073
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Beta-hexosaminidase subunit alpha is involved in catalysis of degradation of the ganglioside GM2. Disfunction of beta-hexosaminidase leads to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. The enzyme is composed of two subunits, alpha and beta. This gene encodes alpha subunit of beta-hexosaminidase. Western blot with this antibody raised against HEXA detected 60 kDa (precursor) and 54 kDa (mature) size bands (PMID: 27682588, PMID: 30341570).

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