Anticorps Monoclonal anti-HEXIM1

HEXIM1 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgM

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

2C8A2

N° de cat : 66311-1-Ig

Synonymes

Hexamethylene bis-acetamide-inducible protein 1, Estrogen down-regulated gene 1 protein, EDG1, CLP1, CLP 1

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Galerie de données de validation

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  WB analysis of Caco-2 using 66311-1-Ig

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 66311-1-Ig (HEXIM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66311-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66311-1-Ig (HEXIM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66311-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66311-1-Ig (HEXIM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules MDA-MB-231, cellules Caco-2, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66311-1-Ig cible HEXIM1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgM
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HEXIM1 Protéine recombinante Ag8019
• Nom complet: hexamethylene bis-acetamide inducible 1
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC006460
• Symbole du gène: HEXIM1
• Identification du gène (NCBI): 10614
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Chromatographie d'affinité thiophilique
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HEXIM1, also named CLP1, EDG1, HIS1 or MAQ1, is best known as the inhibitor of positive transcription elongation factor b, which regulates the transcription elongation of RNA polymerase II and controls 60-70% of mRNA synthesis. HEXIM1 regulates NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Previous studies showed that HEXIM1 interacts with two key p53 regulators, nucleophosmin and HDM2, implying a possible connection between HEXIM1 and the p53 signaling pathway.The mainstream MW of this protein is 65-70 kDa and 54 kDa ( PMID: 33627647, PMID: 20976203, PMID: 28254838).

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