Anticorps Recombinant de lapin anti-HEXIM1

HEXIM1 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242587A2

N° de cat : 85028-1-PBS

Synonymes

242587A2, CLP 1, CLP1, EDG1, Estrogen down-regulated gene 1 protein

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Galerie de données de validation

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  WB analysis using 85028-1-RR (same clone as 85028-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85028-1-RR (HEXIM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85028-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 85028-1-RR (same clone as 85028-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85028-1-RR (HEXIM1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85028-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 85028-1-RR (same clone as 85028-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85028-1-RR (HEXIM1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85028-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 85028-1-RR (same clone as 85028-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HEXIM1 antibody (85028-1-RR, Clone: 242587A2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85028-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 85028-1-RR (same clone as 85028-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug HEXIM1 Recombinant antibody (85028-1-RR, Clone:242587A2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 85028-1-PBS in a different storage buffer formulation.

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Informations sur le produit

85028-1-PBS cible HEXIM1 dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HEXIM1 Protéine recombinante Ag8144
• Nom complet: hexamethylene bis-acetamide inducible 1
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC006460
• Symbole du gène: HEXIM1
• Identification du gène (NCBI): 10614
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HEXIM1, also named CLP1, EDG1, HIS1 or MAQ1, is best known as the inhibitor of positive transcription elongation factor b, which regulates the transcription elongation of RNA polymerase II and controls 60-70% of mRNA synthesis. HEXIM1 regulates NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Previous studies showed that HEXIM1 interacts with two key p53 regulators, nucleophosmin and HDM2, implying a possible connection between HEXIM1 and the p53 signaling pathway.The mainstream MW of this protein is 65-70 kDa and 54 kDa ( PMID: 33627647, PMID: 20976203, PMID: 28254838).

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