Anticorps Polyclonal de lapin anti-HEY1

• Phare
• Validé par KD/KO

HEY1 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, CoIP, chIP, ELISA

Conjugaison

Non conjugué

Publications

43

Avis (1)

N° de cat : 19929-1-AP

Synonymes

BHLHb31, CHF 2, CHF2, HERP2, HESR 1, HESR1, HEY1, hHRT1, HRT 1, HRT1, OAF1

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Galerie de données de validation

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  WB analysis using 19929-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19929-1-AP (HEY1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 19929-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 19929-1-AP (HEY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 19929-1-AP

  IP Result of anti-HEY1 (IP:19929-1-AP, 4ug; Detection:19929-1-AP 1:500) with mouse kidney tissue lysate 4400ug.

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules A549, cellules NCI-H1299, tissu testiculaire de rat
• Résultats positifs en IP: tissu rénal de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 38 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 3 publications below

Informations sur le produit

19929-1-AP cible HEY1 dans les applications de WB, IP, IHC, IF, FC, CoIP, chIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HEY1 Protéine recombinante Ag13906
• Nom complet: hairy/enhancer-of-split related with YRPW motif 1
• Masse moléculaire calculée: 304 aa, 33 kDa
• Poids moléculaire observé: 32-34 kDa
• Numéro d’acquisition GenBank: BC001873
• Symbole du gène: HEY1
• Identification du gène (NCBI): 23462
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Hairy/enhancer of split-related proteins are basic helix-loop-helix (bHLH) transcription factors implicated in cell fate decision and boundary formation. And HEY1 is one of such protein. It is the direct downstream effector of Notch signaling which may be required for cardiovascular development. It preferentially bind to the canonical E box sequence 5'-CACGTG-3' and acts as a transcriptional repressor. HEY1 is a 33kDa protein and forms dimer by self-associating.

Publications

Species	Application	Title
human	WB	J Exp Clin Cancer Res; ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK; Authors - Li Cui
mouse	WB	Dev Cell; Regulation of reverse electron transfer at mitochondrial complex I by unconventional Notch action in cancer stem cells.; Authors - Rani Ojha
human	WB	Nat Commun; Cathepsin K-mediated notch1 activation contributes to neovascularization in response to hypoxia.; Authors - Haiying Jiang
human	WB	Theranostics; Exosome-mediated delivery of RBP-J decoy oligodeoxynucleotides ameliorates hepatic fibrosis in mice.; Authors - Fei He
human	WB	Oncogene; microRNA-23a, -27a and -24 synergistically regulate JAK1/Stat3 cascade and serve as novel therapeutic targets in human acute erythroid leukemia.; Authors - R Su
	WB	Cell Mol Biol Lett; Notch mediates the glycolytic switch via PI3K/Akt signaling to support embryonic development; Authors - Heng Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Zhenminm (Verified Customer) (07-10-2019)

It is too much non-specific background.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: Mouse Testis

HEY1 Antibody Western Blot, validation (1:1,000 dilution) in Mouse Testis (Cat no:19929-1-AP)