Anticorps Polyclonal de lapin anti-HEY2

• Phare
• Validé par KD/KO

HEY2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

42

Avis (3)

N° de cat : 10597-1-AP

Synonymes

bHLHb32, CHF1, GRIDLOCK, GRL, hCHF1, HERP, HERP1, HESR 2, HESR2, HEY2, hHRT2, HRT 2, HRT2, Protein gridlock homolog

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Galerie de données de validation

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  WB analysis of K-562 using 10597-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10597-1-AP (HEY2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10597-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10597-1-AP (HEY2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 4T1 using 10597-1-AP

  4T1 cells were subjected to SDS PAGE followed by western blot with 10597-1-AP (HEY2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10597-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10597-1-AP (HEY2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10597-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10597-1-AP (HEY2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 10597-1-AP

  IP Result of anti-HEY2 (IP:10597-1-AP, 5ug; Detection:10597-1-AP 1:500) with mouse heart tissue lysate 7500ug.

• 

  IHC staining of human prostate cancer using 10597-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10597-1-AP (HEY2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HepG2 using 10597-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10597-1-AP (HEY2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 10597-1-AP

  Immunofluorescent analysis of HepG2 cells, using HEY2 antibody 10597-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules 4T1, cellules MCF-7, tissu cardiaque de souris
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 25 publications below
• IHC: See 9 publications below
• IF: See 8 publications below
• CoIP: See 1 publications below
• ChIP: See 2 publications below

Informations sur le produit

10597-1-AP cible HEY2 dans les applications de WB, IP, IHC, IF, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HEY2 Protéine recombinante Ag0913
• Nom complet: hairy/enhancer-of-split related with YRPW motif 2
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC007707
• Symbole du gène: HEY2
• Identification du gène (NCBI): 23493
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Hairy/enhancer of split-related proteins, such as HEY2, are basic helix-loop-helix (bHLH) transcription factors implicated in cell fate decision and boundary formation. HEY genes are direct transcriptional targets of the Notch signaling pathways in Drosophila and vertebrates. HEY2 is similarly expressed in the somites whereas it shows a complementary expression in the heart, the craniofacial region and the nervous system. This antibody is a rabbit polyclonal antibody raised against full length of human HEY2

Publications

Species	Application	Title
human	IF	Cell; Multi-chamber cardioids unravel human heart development and cardiac defects; Authors - Clara Schmidt
human	IF	Cell Stem Cell; Generation of Functional Liver Sinusoidal Endothelial Cells from Human Pluripotent Stem-Cell-Derived Venous Angioblasts.; Authors - Blair K Gage
mouse	WB	Nat Neurosci; Zeb2 recruits HDAC-NuRD to inhibit Notch and controls Schwann cell differentiation and remyelination.; Authors - Lai Man Natalie Wu
human	chIP	Nat Commun; Defining human cardiac transcription factor hierarchies using integrated single-cell heterogeneity analysis.; Authors - Jared M Churko
human	IF	Nat Commun; Generation of mature compact ventricular cardiomyocytes from human pluripotent stem cells.; Authors - Shunsuke Funakoshi
human	WB	Nat Commun; Cathepsin K-mediated notch1 activation contributes to neovascularization in response to hypoxia.; Authors - Haiying Jiang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

CHAO (Verified Customer) (03-31-2022)

Weak band around correct size.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Heart

FH

Tom (Verified Customer) (08-22-2020)

I performed western blot with mouse heart tissue lysate using Hey2 antibody. A specific ~36 kDa Hey2 band was observed.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart

FH

Tom (Verified Customer) (08-22-2020)

This Hey2 antibody is highly recommended for detecting Hey2 on frozen tissue sections! I used this Hey2 antibody for immunofluorescence (IF). It clearly had nuclear staining signal in compact myocardium of embryonic heart, which is consistent with Hey2's well-known expression pattern.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse embryonic heart

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