Anticorps Recombinant de lapin anti-HEY2

HEY2 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240474F2

N° de cat : 83501-7-PBS

Synonymes

GRIDLOCK, Class B basic helix-loop-helix protein 32, CHF1, Cardiovascular helix-loop-helix factor 1, bHLHb32

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Galerie de données de validation

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  IF Staining of HeLa using 83501-7-RR (same clone as 83501-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HEY2 antibody (83501-7-RR, Clone: 240474F2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83501-7-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83501-7-RR (same clone as 83501-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HEY2 antibody (83501-7-RR, Clone: 240474F2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83501-7-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 83501-7-RR (same clone as 83501-7-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug HEY2 Recombinant antibody (83501-7-RR, Clone:240474F2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Control Antibody (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83501-7-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83501-7-RR (same clone as 83501-7-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug HEY2 Recombinant antibody (83501-7-RR, Clone:240474F2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83501-7-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83501-7-RR

  Biolayer interferometry (BLl) kinetic assays of 83501-7-RR against Human HEY2 were performed. The affinity constant is 1.08 nM.

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Informations sur le produit

83501-7-PBS cible HEY2 dans les applications de IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HEY2 Protéine recombinante Ag30988
• Nom complet: hairy/enhancer-of-split related with YRPW motif 2
• Masse moléculaire calculée: 36 kDa
• Numéro d’acquisition GenBank: BC007707
• Symbole du gène: HEY2
• Identification du gène (NCBI): 23493
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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