Anticorps Recombinant de lapin anti-HGD

HGD Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242485E11

N° de cat : 84931-5-PBS

Synonymes

242485E11, AKU, EC:1.13.11.5, HGO, Homogentisate 1,2 dioxygenase

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Galerie de données de validation

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  WB analysis using 84931-5-RR (same clone as 84931-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84931-5-RR (HGD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84931-5-PBS in a different storage buffer formulation.

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  WB analysis using 84931-5-RR (same clone as 84931-5-PBS)

  rat liver tissue lysates were subjected to SDS PAGE followed by western blot with 84931-5-RR (HGD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84931-5-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 84931-5-RR (same clone as 84931-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 84931-5-RR (HGD antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84931-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84931-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84931-5-RR against Human HGD were performed. The affinity constant is 1.38 nM.

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Informations sur le produit

84931-5-PBS cible HGD dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HGD Protéine recombinante Ag9544
• Nom complet: homogentisate 1,2-dioxygenase (homogentisate oxidase)
• Masse moléculaire calculée: 37 kDa, 50 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC020792
• Symbole du gène: HGD
• Identification du gène (NCBI): 3081
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Homogentisate1,2-dioxygenase (HGD), also named as HGO, is a mononuclear Fe(II)-dependent oxygenase that catalyzes the third step in the pathway for the catabolism of tyrosine, the conversion of homogentisate (HG) to maleylacetoacetate (MAA) and it can exsit as a dimer or trimer(PMID:14678794). HGD consists of a single type of subunit with no intermolecular disulfide bridges and requires Fe2+ as a cofactor(PMID: 7705358). Defects in HGD are the cause of alkaptonuria (AKU)(PMID:10594001).

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