Anticorps Polyclonal de lapin anti-HGS

• Phare
• Validé par KD/KO

HGS Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 10390-1-AP

Synonymes

HGS, HRS, Protein pp110, ZFYVE8

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Galerie de données de validation

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  WB analysis using 10390-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10390-1-AP (HGS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10390-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10390-1-AP (HGS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 10390-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10390-1-AP (HGS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10390-1-AP

  IP Result of anti-HGS (IP:10390-1-AP, 5ug; Detection:10390-1-AP 1:1000) with mouse brain tissue lysate 3000ug.

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  IHC staining of human liver using 10390-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10390-1-AP (HGS Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 10390-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10390-1-AP (HGS Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse brain using 10390-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10390-1-AP (HGS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10390-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10390-1-AP (HGS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 10390-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HGS antibody (10390-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules K-562, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu hépatique humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Informations sur le produit

10390-1-AP cible HGS dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HGS Protéine recombinante Ag0589
• Nom complet: hepatocyte growth factor-regulated tyrosine kinase substrate
• Masse moléculaire calculée: 86 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC003565
• Symbole du gène: HGS
• Identification du gène (NCBI): 9146
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Hepatocyte growth factor-regulated tyrosine kinase substrate (HGS, synonyms: HRS, ZFYVE8) is a 110 to 115-kDa zinc finger phosphotyrosine protein inducible by stimulation with interleukin 2 (IL-2), granulocyte-macrophage colony-stimulating factor (GM-CSF) as well as hepatocyte growth factor (HGF), and is associated with signal-transducing adaptor molecule (STAM). HGS suppresses DNA synthesis upon stimulation with IL-2 and GM-CSF, counteracting the function of STAM which is critical for cell growth signaling mediated by the cytokines. HGS also interacts with the neurofibromatosis 2 tumor suppressor protein schwannomin/merlin. The growth suppression activity of schwannomin/merlin requires HGS and the binding of schwannomin/merlin to HGS facilitates its ability to function as a tumor suppressor, probably by inhibiting STAT activation.

Publications

Species	Application	Title
human	WB	Cell Res; RAB31 marks and controls an ESCRT-independent exosome pathway.; Authors - Denghui Wei
human	IHC	Sci Rep; β-defensin 1 expression in HCV infected liver/liver cancer: an important role in protecting HCV progression and liver cancer development.; Authors - Yue-Ming Ling
human	WB,IF	Mol Biol Cell; The ubiquitin ligase Deltex-3L regulates endosomal sorting of the G protein-coupled receptor CXCR4.; Authors - Justine Holleman
human	IF	J Cell Sci; Neutral sphingomyelinase 2 controls exosome secretion by counteracting V-ATPase-mediated endosome acidification.; Authors - Dolma Choezom
human	IF	iScience; VPS28 regulates brain vasculature by controlling neuronal VEGF trafficking through extracellular vesicle secretion.; Authors - Xiaohua Dong
human	IF	iScience; Compartmentalization of casein kinase 1 γ CSNK1G controls the intracellular trafficking of ceramide.; Authors - Asako Goto

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