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  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-HGS

HGS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(15)

N° de cat : 10390-1-AP

Synonymes

ZFYVE8, Protein pp110, Hrs, Hepatocyte growth factor-regulated tyrosine kinase substrate



Applications testées

Résultats positifs en WBcellules HeLa, cellules K-562, tissu cérébral de rat, tissu cérébral de souris
Résultats positifs en IPtissu cérébral de souris
Résultats positifs en IHCtissu hépatique humain, tissu cérébral de souris
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF/ICCcellules HeLa,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochimie (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

10390-1-AP cible HGS dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéeHumain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène HGS Protéine recombinante Ag0589
Nom complet hepatocyte growth factor-regulated tyrosine kinase substrate
Masse moléculaire calculée 86 kDa
Poids moléculaire observé 110 kDa
Numéro d’acquisition GenBankBC003565
Symbole du gène HGS
Identification du gène (NCBI) 9146
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Hepatocyte growth factor-regulated tyrosine kinase substrate (HGS, synonyms: HRS, ZFYVE8) is a 110 to 115-kDa zinc finger phosphotyrosine protein inducible by stimulation with interleukin 2 (IL-2), granulocyte-macrophage colony-stimulating factor (GM-CSF) as well as hepatocyte growth factor (HGF), and is associated with signal-transducing adaptor molecule (STAM). HGS suppresses DNA synthesis upon stimulation with IL-2 and GM-CSF, counteracting STAM's function, which is critical for cell growth signaling mediated by the cytokines. HGS also interacts with the neurofibromatosis 2 tumor suppressor protein Schwannomin/merlin. The growth suppression activity of schwannoma/merlin requires HGS. The binding of schwannoma/merlin to HGS facilitates its ability to function as a tumor suppressor, probably by inhibiting STAT activation.

Protocole

Product Specific Protocols
WB protocol for HGS antibody 10390-1-APDownload protocol
IHC protocol for HGS antibody 10390-1-APDownload protocol
IF protocol for HGS antibody 10390-1-APDownload protocol
IP protocol for HGS antibody 10390-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Res

RAB31 marks and controls an ESCRT-independent exosome pathway.

Authors - Denghui Wei
humanWB

EMBO Rep

The USP12/46 deubiquitinases protect integrins from ESCRT-mediated lysosomal degradation

Authors - Kaikai Yu
humanIHC

Sci Rep

β-defensin 1 expression in HCV infected liver/liver cancer: an important role in protecting HCV progression and liver cancer development.

Authors - Yue-Ming Ling
humanWB,IF

Mol Biol Cell

The ubiquitin ligase Deltex-3L regulates endosomal sorting of the G protein-coupled receptor CXCR4.

Authors - Justine Holleman
humanIF

J Cell Sci

Neutral sphingomyelinase 2 controls exosome secretion by counteracting V-ATPase-mediated endosome acidification.

Authors - Dolma Choezom
Human

J Virol

Human Cytomegalovirus Hijacks WD Repeat Domain 11 for Virion Assembly Compartment Formation and Virion Morphogenesis.

Authors - Bo Yang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Wojciech (Verified Customer) (09-10-2025)

Tested HG5 antibody in T-cells and it consistently detected the expected 100 kDa band with strong specificity and very low background. Results were reproducible across experiments, and overall performance was good.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: leukemic T-cells
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