- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-HGS
HGS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugaison
Non conjugué
N° de cat : 10390-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules HeLa, cellules K-562, tissu cérébral de rat, tissu cérébral de souris |
Résultats positifs en IP | tissu cérébral de souris |
Résultats positifs en IHC | tissu hépatique humain, tissu cérébral de souris il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
Résultats positifs en IF/ICC | cellules HeLa, |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochimie (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
KD/KO | See 1 publications below |
WB | See 10 publications below |
IHC | See 1 publications below |
IF | See 6 publications below |
Informations sur le produit
10390-1-AP cible HGS dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
Réactivité | Humain, rat, souris |
Réactivité citée | Humain, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | HGS Protéine recombinante Ag0589 |
Nom complet | hepatocyte growth factor-regulated tyrosine kinase substrate |
Masse moléculaire calculée | 86 kDa |
Poids moléculaire observé | 110 kDa |
Numéro d’acquisition GenBank | BC003565 |
Symbole du gène | HGS |
Identification du gène (NCBI) | 9146 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Hepatocyte growth factor-regulated tyrosine kinase substrate (HGS, synonyms: HRS, ZFYVE8) is a 110 to 115-kDa zinc finger phosphotyrosine protein inducible by stimulation with interleukin 2 (IL-2), granulocyte-macrophage colony-stimulating factor (GM-CSF) as well as hepatocyte growth factor (HGF), and is associated with signal-transducing adaptor molecule (STAM). HGS suppresses DNA synthesis upon stimulation with IL-2 and GM-CSF, counteracting STAM's function, which is critical for cell growth signaling mediated by the cytokines. HGS also interacts with the neurofibromatosis 2 tumor suppressor protein Schwannomin/merlin. The growth suppression activity of schwannoma/merlin requires HGS. The binding of schwannoma/merlin to HGS facilitates its ability to function as a tumor suppressor, probably by inhibiting STAT activation.
Protocole
Product Specific Protocols | |
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WB protocol for HGS antibody 10390-1-AP | Download protocol |
IHC protocol for HGS antibody 10390-1-AP | Download protocol |
IF protocol for HGS antibody 10390-1-AP | Download protocol |
IP protocol for HGS antibody 10390-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
EMBO Rep The USP12/46 deubiquitinases protect integrins from ESCRT-mediated lysosomal degradation | ||
Sci Rep β-defensin 1 expression in HCV infected liver/liver cancer: an important role in protecting HCV progression and liver cancer development. | ||
Mol Biol Cell The ubiquitin ligase Deltex-3L regulates endosomal sorting of the G protein-coupled receptor CXCR4. | ||
J Cell Sci Neutral sphingomyelinase 2 controls exosome secretion by counteracting V-ATPase-mediated endosome acidification. | ||
J Virol Human Cytomegalovirus Hijacks WD Repeat Domain 11 for Virion Assembly Compartment Formation and Virion Morphogenesis. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Wojciech (Verified Customer) (09-10-2025) | Tested HG5 antibody in T-cells and it consistently detected the expected 100 kDa band with strong specificity and very low background. Results were reproducible across experiments, and overall performance was good.
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