Anticorps Polyclonal de lapin anti-HHIP

HHIP Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29466-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 29466-1-AP (same clone as 29466-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29466-1-AP (HHIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29466-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 29466-1-AP (same clone as 29466-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 29466-1-AP (HHIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29466-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 29466-1-AP (same clone as 29466-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29466-1-AP (HHIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29466-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 29466-1-AP (same clone as 29466-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29466-1-AP (HHIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29466-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 29466-1-AP (same clone as 29466-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29466-1-AP (HHIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29466-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 29466-1-AP (same clone as 29466-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 29466-1-AP (HHIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29466-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 29466-1-AP (same clone as 29466-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HHIP antibody (29466-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29466-1-PBS in a different storage buffer formulation.

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Informations sur le produit

29466-1-PBS cible HHIP dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HHIP Protéine recombinante Ag30869
• Nom complet: hedgehog interacting protein
• Masse moléculaire calculée: 700 aa, 79 kDa
• Poids moléculaire observé: 85 kDa
• Numéro d’acquisition GenBank: BC025311
• Symbole du gène: HHIP
• Identification du gène (NCBI): 64399
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The hedgehog (HH) proteins are evolutionarily conserved protein, which are important morphogens for a wide range of developmental processes, including anteroposterior patterns of limbs and regulation of left-right asymmetry in embryonic development. Multiple cell-surface receptors are responsible for transducing and/or regulating HH signals. HHIP is a highly conserved, vertebrate-specific inhibitor of HH signaling. It interacts with all three HH family members, SHH, IHH and DHH.

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