Anticorps Monoclonal anti-HIBCH

• Phare
• Validé par KD/KO

HIBCH Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B7B8

N° de cat : 68157-1-PBS

Synonymes

HIBYL COA H, HIB-CoA hydrolase, HIB CoA hydrolase, EC:3.1.2.4, 3-hydroxyisobutyryl-coenzyme A hydrolase

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Galerie de données de validation

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  WB analysis using 68157-1-Ig (same clone as 68157-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68157-1-Ig (HIBCH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68157-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 68157-1-Ig (same clone as 68157-1-PBS)

  WB result of HIBCH antibody (68157-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIBCH transfected HepG2 cells. This data was developed using the same antibody clone with 68157-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68157-1-Ig (same clone as 68157-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HIBCH antibody (68157-1-Ig, Clone: 1B7B8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68157-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68157-1-PBS cible HIBCH dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HIBCH Protéine recombinante Ag6473
• Nom complet: 3-hydroxyisobutyryl-Coenzyme A hydrolase
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC067822
• Symbole du gène: HIBCH
• Identification du gène (NCBI): 26275
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HIBCH belongs to the enoyl-CoA hydratase/isomerase family. HIBCH has two isoforms with MW 43 kDa and 38 kDa. It is highly expressed in the liver and kidney, also detected in the heart, muscle and brain (at protein level), but not detected in the lung. Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA (PMID: 8824301).

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