Anticorps Recombinant de lapin anti-HIBCH

• Phare
• Validé par KD/KO

HIBCH Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

240590A3

N° de cat : 83569-1-PBS

Synonymes

240590A3, 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial, 3-hydroxyisobutyryl-coenzyme A hydrolase, EC:3.1.2.4, HIB CoA hydrolase

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Galerie de données de validation

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  WB analysis using 83569-1-RR (same clone as 83569-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83569-1-RR (HIBCH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83569-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 83569-1-RR (same clone as 83569-1-PBS)

  WB result of HIBCH antibody (83569-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIBCH transfected HepG2 cells. This data was developed using the same antibody clone with 83569-1-PBS in a different storage buffer formulation.

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  Sample test of MP00560-3

  The mean HIBCH concentration was determined to be 24.8 ng/mL in HepG2 cell extract based on a 1.3 mg/mL extract load.

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  IF Staining of HepG2 using 83569-1-RR (same clone as 83569-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HIBCH antibody (83569-1-RR, Clone: 240590A3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 83569-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83569-1-RR (same clone as 83569-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIBCH antibody (83569-1-RR, Clone: 240590A3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 83569-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 83569-1-RR (same clone as 83569-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug HIBCH Recombinant antibody (83569-1-RR, Clone:240590A3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83569-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00560-3

  Cytometric bead array standard curve of MP00560-3, HIBCH Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83569-3-PBS. Detection antibody: 83569-1-PBS. Standard: Ag6149. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 83569-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83569-1-RR against Human HIBCH were performed. The affinity constant is 1.37 nM.

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Informations sur le produit

83569-1-PBS cible HIBCH dans les applications de WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HIBCH Protéine recombinante Ag6149
• Nom complet: 3-hydroxyisobutyryl-Coenzyme A hydrolase
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC067822
• Symbole du gène: HIBCH
• Identification du gène (NCBI): 26275
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HIBCH belongs to the enoyl-CoA hydratase/isomerase family. HIBCH has two isoforms with MW 43 kDa and 38 kDa. It is highly expressed in the liver and kidney, also detected in the heart, muscle and brain (at protein level), but not detected in the lung. Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA (PMID: 8824301).

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