Anticorps Polyclonal de lapin anti-HIC5

• Phare
• Validé par KD/KO

HIC5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10565-1-PBS

Synonymes

Androgen receptor-associated protein of 55 kDa, ARA55, Hic-5, Hydrogen peroxide-inducible clone 5 protein, TGFB1I1

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Galerie de données de validation

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  WB analysis using 10565-1-AP (same clone as 10565-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10565-1-AP (HIC5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10565-1-PBS in a different storage buffer formulation.

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  WB analysis using 10565-1-AP (same clone as 10565-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10565-1-AP (HIC5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10565-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 10565-1-AP (same clone as 10565-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 10565-1-AP (HIC5 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10565-1-PBS in a different storage buffer formulation.

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  IP experiment of A431 using 10565-1-AP (same clone as 10565-1-PBS)

  IP result of anti-HIC5 (IP:10565-1-AP, 4ug; Detection:10565-1-AP 1:500) with A431 cells lysate 1520ug. This data was developed using the same antibody clone with 10565-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 10565-1-AP (same clone as 10565-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 10565-1-AP (HIC5 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10565-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 10565-1-AP (same clone as 10565-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 10565-1-AP (HIC5 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10565-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 10565-1-AP (same clone as 10565-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using HIC5 antibody (10565-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10565-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 10565-1-AP (same clone as 10565-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using HIC5 antibody (10565-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10565-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10565-1-PBS cible HIC5 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HIC5 Protéine recombinante Ag0868
• Nom complet: transforming growth factor beta 1 induced transcript 1
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48-50 kDa
• Numéro d’acquisition GenBank: BC001830
• Symbole du gène: HIC5
• Identification du gène (NCBI): 7041
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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