Anticorps Polyclonal de lapin anti-HIF-1 alpha

• Phare
• Validé par KD/KO

HIF-1 alpha Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 20960-1-PBS

Synonymes

HIF1A, HIF 1α, HIF 1 alpha, Class E basic helix-loop-helix protein 78, Basic-helix-loop-helix-PAS protein MOP1

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Galerie de données de validation

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  WB analysis using 20960-1-AP (same clone as 20960-1-PBS)

  Untreated and Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 20960-1-AP (HIF1a antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20960-1-PBS in a different storage buffer formulation.

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  WB analysis using 20960-1-AP (same clone as 20960-1-PBS)

  Untreated and Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 20960-1-AP (HIF1a antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20960-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 20960-1-AP (same clone as 20960-1-PBS)

  WB result of HIF-1 alpha antibody (20960-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIF-1 alpha transfected HeLa cells. Sample 1: non-treated sh-Control transfected HeLa cells, Sample 2: Cobalt Chloride treated sh-Control transfected HeLa cells, Sample 3: Cobalt Chloride treated sh-HIF-1 alpha transfected HeLa cells. This data was developed using the same antibody clone with 20960-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 20960-1-AP (same clone as 20960-1-PBS)

  Untreated and Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 20960-1-AP (HIF1a antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20960-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 20960-1-AP (same clone as 20960-1-PBS)

  IP result of anti-HIF-1 alpha (IP:20960-1-AP, 4ug; Detection:20960-1-AP 1:300) with HeLa cells lysate 4000ug. This data was developed using the same antibody clone with 20960-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 20960-1-AP (same clone as 20960-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 20960-1-AP (HIF-1 alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20960-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 20960-1-AP (same clone as 20960-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 20960-1-AP (HIF-1 alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20960-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 20960-1-AP (same clone as 20960-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 20960-1-AP (HIF1A antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20960-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 20960-1-AP (same clone as 20960-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 20960-1-AP (HIF1A antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20960-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 20960-1-AP (same clone as 20960-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 20960-1-AP (HIF1A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20960-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 20960-1-AP (same clone as 20960-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 20960-1-AP (HIF1A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20960-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 20960-1-AP (same clone as 20960-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer using 20960-1-AP (HIF1A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20960-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 20960-1-AP (same clone as 20960-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer using 20960-1-AP (HIF1A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20960-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 20960-1-AP (same clone as 20960-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HeLa cells using HIF-1 alpha antibody (20960-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 20960-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 20960-1-AP (same clone as 20960-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed Cobalt Chloride treated HepG2 cells using HIF1a antibody (20960-1-AP) at dilution of 1:1800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20960-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 20960-1-AP (same clone as 20960-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed Cobalt Chloride treated HepG2 cells using HIF1a antibody (20960-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20960-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 20960-1-AP (same clone as 20960-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HeLa cells using HIF-1 alpha antibody (20960-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 20960-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20960-1-PBS cible HIF-1 alpha dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HIF-1 alpha Protéine recombinante Ag15198
• Nom complet: hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
• Masse moléculaire calculée: 826 aa, 93 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC012527
• Symbole du gène: HIF1A
• Identification du gène (NCBI): 3091
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .

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