Anticorps Monoclonal anti-HIF-1 alpha

• Phare
• Validé par KD/KO

HIF-1 alpha Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1H3C12

N° de cat : 66730-1-PBS

Synonymes

HIF1A, 1H3C12, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1

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Galerie de données de validation

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  WB analysis of HeLa using 66730-1-Ig (same clone as 66730-1-PBS)

  Untreated and cobalt chloride treated HeLa and HepG2 cells were subjected to SDS-PAGE followed by western blot with 66730-1-Ig (HIF1a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with YY1 antibody as loading control. This data was developed using the same antibody clone with 66730-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66730-1-Ig (same clone as 66730-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66730-1-Ig (HIF-1 alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66730-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66730-1-Ig (same clone as 66730-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66730-1-Ig (HIF-1 alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66730-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66730-1-Ig (same clone as 66730-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66730-1-Ig (HIF-1 alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66730-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66730-1-Ig (same clone as 66730-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66730-1-Ig (HIF-1 alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66730-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66730-1-PBS cible HIF-1 alpha dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HIF-1 alpha Protéine recombinante Ag15198
• Nom complet: hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
• Masse moléculaire calculée: 826 aa, 93 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC012527
• Symbole du gène: HIF1A
• Identification du gène (NCBI): 3091
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .

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