Anticorps Monoclonal anti-HIF-1 alpha

• Phare
• Validé par KD/KO

HIF-1 alpha Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF, IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(72)

CloneNo.

1H3C12

Avis (3)

N° de cat : 66730-1-Ig

Synonymes

HIF1A, 1H3C12, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1

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Galerie de données de validation

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  WB analysis of HeLa using 66730-1-Ig

  Untreated and cobalt chloride treated HeLa and HepG2 cells were subjected to SDS-PAGE followed by western blot with 66730-1-Ig (HIF1a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with YY1 antibody as loading control.

• 

  IHC staining of human ovary tumor using 66730-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66730-1-Ig (HIF-1 alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66730-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66730-1-Ig (HIF-1 alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66730-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66730-1-Ig (HIF-1 alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 66730-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66730-1-Ig (HIF-1 alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HeLa traitées au chlorure de cobalt, cellules HepG2 traitées au chlorure de cobalt
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 61 publications below
• IHC: See 15 publications below
• IF: See 17 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

66730-1-Ig cible HIF-1 alpha dans les applications de WB, IHC, IF, IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HIF-1 alpha Protéine recombinante Ag15198
• Nom complet: hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
• Masse moléculaire calculée: 826 aa, 93 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC012527
• Symbole du gène: HIF1A
• Identification du gène (NCBI): 3091
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .

Publications

Species	Application	Title
human	WB,IP,IHC,IF	Adv Sci (Weinh); Transgelin Promotes Glioblastoma Stem Cell Hypoxic Responses and Maintenance Through p53 Acetylation; Authors - Huan Li
	IF	Cell Rep Med; Ultrasound-visible engineered bacteria for tumor chemo-immunotherapy; Authors - Yaozhang Yang
human	WB	J Immunother Cancer; TSH-TSHR axis promotes tumor immune evasion.; Authors - Zhenghao Wu
human	WB	Theranostics; ITGB2-mediated metabolic switch in CAFs promotes OSCC proliferation by oxidation of NADH in mitochondrial oxidative phosphorylation system.; Authors - Xiaoxin Zhang
	WB	Phytomedicine; mTOR/HIF-1α pathway-mediated glucose reprogramming and macrophage polarization by Sini decoction plus ginseng soup in ALF; Authors - Junli Zhang
human	WB	Oxid Med Cell Longev; Chitosan Oligosaccharides Alleviate H2O2-stimulated Granulosa Cell Damage via HIF-1α Signaling Pathway.; Authors - Ziwei Yang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

S (Verified Customer) (05-16-2024)

Excellent

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: AC16

HIF-1 alpha Antibody Western Blot validation (1000 dilution) in AC16 (Cat no:66730-1-Ig)

FH

balawant (Verified Customer) (12-20-2022)

I have used this antibody for western blot and it is working great

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: renal Cancer cell line

FH

Diane (Verified Customer) (02-02-2021)

incubated over night 4 degrees C. 1:2500 HRP conjugated secondary. Opti-4CN substrate kit for detection. Also tried ICC on cytospins so ran out of trial size before fully optimized but has potential to provide the information that I am needing.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1250
• Cell Tissue Type: Cell lysates glioblastoma primary cells

HIF-1 alpha Antibody Western Blot validation (1:1250 dilution) in Cell lysates glioblastoma primary cells (Cat no:66730-1-Ig)

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