Anticorps Recombinant de lapin anti-HIF-1 alpha

• Phare
• Validé par KD/KO

HIF-1 alpha Recombinant Antibody for WB, IF/ICC, ELISA, ChIP-qPCR

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA, ChIP-qPCR

Conjugaison

Non conjugué

Publications(1)

CloneNo.

2K1

N° de cat : 80933-1-RR

Synonymes

HIF1A, 2K1, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1

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Galerie de données de validation

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  WB analysis of HeLa using 80933-1-RR

  Untreated and Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 80933-1-RR (HIF1a antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 80933-1-RR

  WB result of HIF-1 alpha antibody (80933-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIF-1 alpha transfected HeLa cells. Sample 1: non-treated sh-Control transfected HeLa cells, Sample 2: Cobalt Chloride treated sh-Control transfected HeLa cells, Sample 3: Cobalt Chloride treated sh-HIF-1 alpha transfected HeLa cells.

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  IF Staining of HepG2 using 80933-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HepG2 cells using HIF1a antibody (80933-1-RR, Clone: 2K1 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 594+phalloidin (red).

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  IF Staining of HepG2 using 80933-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HepG2 cells using HIF1a antibody (80933-1-RR, Clone: 2K1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 80933-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HeLa cells using HIF-1 alpha antibody (80933-1-RR, Clone: 2K1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  ChIP experiment of HeLa using 80933-1-RR

  Chromatin was prepared from HeLa cells treated with cobalt chloride (100 μM, overnight). Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of HIF-1 alpha (80933-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

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  Affinity and Kinetic Characterization of 80933-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80933-1-RR against Human HIF-1 alpha were performed. The affinity constant is 0.235 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa traitées au chlorure de cobalt, cellules HeLa
• Résultats positifs en IF/ICC: cellules HeLa traitées au chlorure de cobalt, cellules HepG2 traitées au chlorure de cobalt
• Résultats positifs en ChIP-qPCR: cobalt chloride (100 μM, overnight) HeLa cells,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• CHImmunoprécipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

80933-1-RR cible HIF-1 alpha dans les applications de WB, IHC, IF/ICC, ELISA, ChIP-qPCR et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HIF-1 alpha Protéine recombinante Ag15198
• Nom complet: hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
• Masse moléculaire calculée: 826 aa, 93 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC012527
• Symbole du gène: HIF1A
• Identification du gène (NCBI): 3091
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, PMID: 7539918).

Publications

Species	Application	Title
human	IHC	Int Immunopharmacol; Unraveling the crucial role of SDF-1 in osteoarthritis progression: IL6/HIF-1α positive feedback and chondrocyte ferroptosis; Authors - Tengyun Yang

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