Anticorps Polyclonal de lapin anti-HIF3A

• Phare
• Validé par KD/KO

HIF3A Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 27650-1-AP

Synonymes

HIF3 alpha, HIF 3A4, HIF 3A2, HIF 3A, HIF 3 alpha

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Galerie de données de validation

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  WB analysis of A431 using 27650-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 27650-1-AP (HIF3A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 27650-1-AP

  Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 27650-1-AP (HIF3A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 27650-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27650-1-AP (HIF3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 27650-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Tunicamycin treated HeLa cells using HIF3A antibody (27650-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HeLa traitées au chlorure de cobalt, cellules A431
• Résultats positifs en IF/ICC: cellules HeLa traitées à la tunicamycine,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 3 publications below

Informations sur le produit

27650-1-AP cible HIF3A dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HIF3A Protéine recombinante Ag26574
• Nom complet: hypoxia inducible factor 3, alpha subunit
• Masse moléculaire calculée: 72 kDa
• Poids moléculaire observé: 72 kDa
• Numéro d’acquisition GenBank: NM_022462
• Symbole du gène: HIF3A
• Identification du gène (NCBI): 64344
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HIF3A, Hypoxia-inducible factor 3-alpha, acts as a transcriptional regulator in adaptive response to low oxygen tension (PMID:11573933, PMID:16126907, PMID:19694616, PMID:20416395, PMID:21069422). It functions as an inhibitor of angiogenesis in hypoxic cells of the cornea. HIF3A also plays a role in the development of the cardiorespiratory system. HIF3A was up-regulated by hypoxia (PMID:16775626). Multiple alternatively spliced transcript variants have been found.

Publications

Species	Application	Title
human	WB	Nat Commun; Identification of oleoylethanolamide as an endogenous ligand for HIF-3α.; Authors - Xiaotong Diao
human	WB	Mol Metab; Genome-wide association study of adipocyte lipolysis in the GENetics of adipocyte lipolysis (GENiAL) cohort.; Authors - Agné Kulyté ; KD Validated
mouse	WB	Front Cell Dev Biol; HIF3A Inhibition Triggers Browning of White Adipocytes via Metabolic Rewiring.; Authors - Francesca Cuomo ; KD Validated
mouse	WB	Toxicol Lett; Knockdown of long noncoding RNA MIAT attenuates cigarette smoke-induced airway remodeling by downregulating miR-29c-3p-HIF3A axis.; Authors - Wenchao Gu
human	IHC	ERJ Open Res; Dynamic analysis of gene signatures in the progression of COPD; Authors - Junchao Jiang
mouse	IHC	Bioact Mater; Synergistic rheumatoid arthritis therapy by interrupting the detrimental feedback loop to orchestrate hypoxia M1 macrophage polarization using an enzyme-catalyzed nanoplatform; Authors - Dong Guo

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