Anticorps Polyclonal de lapin anti-HIGD1A

• Phare
• Validé par KD/KO

HIGD1A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 21749-1-AP

Synonymes

HIG1, HIG1 domain family member 1A, HIG1 domain family member 1A, mitochondrial, HSPC010, Hypoxia-inducible gene 1 protein

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Galerie de données de validation

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  WB analysis using 21749-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21749-1-AP (HIGD1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21749-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21749-1-AP (HIGD1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse ovary using 21749-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 21749-1-AP (HIGD1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 21749-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 21749-1-AP (HIGD1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 21749-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HIGD1A antibody (21749-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules HepG2
• Résultats positifs en IHC: tissu ovarien de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 8 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Informations sur le produit

21749-1-AP cible HIGD1A dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HIGD1A Protéine recombinante Ag14027
• Nom complet: HIG1 hypoxia inducible domain family, member 1A
• Masse moléculaire calculée: 93 aa, 10 kDa
• Poids moléculaire observé: 10 kDa
• Numéro d’acquisition GenBank: BC000601
• Symbole du gène: HIGD1A
• Identification du gène (NCBI): 25994
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HIG1 domain family member 1A (HIGD1A) is a proposed subunit of cytochrome c oxidase (COX, complex IV), which is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. May play a role in the assembly of respiratory supercomplexes (22342701). It is induced by hypoxia induction.This antibody is a rabbit polyclonal antibody raised against a full-length human HIGD1A protein.

Publications

Species	Application	Title
human	WB,IP,IF	Proc Natl Acad Sci U S A; Higd1a is a positive regulator of cytochrome c oxidase.; Authors - Takaharu Hayashi ; KD Validated
human	WB,IP,IF	Oncogene; DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity.; Authors - Bin Chen ; KD Validated
human	WB	Mol Cell Proteomics; HIGD2A is Required for Assembly of the COX3 Module of Human Mitochondrial Complex IV; Authors - Daniella H Hock ; KO Validated
human	WB	FASEB J; Higd1a improves respiratory function in the models of mitochondrial disorder.; Authors - Takemasa Nagao
human	WB	Mol Cell Proteomics; HIGD2A is Required for Assembly of the COX3 Module of Human Mitochondrial Complex IV.; Authors - Daniella H Hock ; KO Validated
	WB,IF	PLoS One; Nuclear localization of the mitochondrial factor HIGD1A during metabolic stress.; Authors - Ameri Kurosh K

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