Anticorps Polyclonal de lapin anti-HIGD1A

• Phare
• Validé par KD/KO

HIGD1A Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21749-1-PBS

Synonymes

HIG1, HIG1 domain family member 1A, HIG1 domain family member 1A, mitochondrial, HSPC010, Hypoxia-inducible gene 1 protein

---

Galerie de données de validation

• 

  WB analysis using 21749-1-AP (same clone as 21749-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21749-1-AP (HIGD1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21749-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 21749-1-AP (same clone as 21749-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 21749-1-AP (HIGD1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21749-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 21749-1-AP (same clone as 21749-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HIGD1A antibody (21749-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21749-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

21749-1-PBS cible HIGD1A dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HIGD1A Protéine recombinante Ag14027
• Nom complet: HIG1 hypoxia inducible domain family, member 1A
• Masse moléculaire calculée: 93 aa, 10 kDa
• Poids moléculaire observé: 10 kDa
• Numéro d’acquisition GenBank: BC000601
• Symbole du gène: HIGD1A
• Identification du gène (NCBI): 25994
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HIG1 domain family member 1A (HIGD1A) is a proposed subunit of cytochrome c oxidase (COX, complex IV), which is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. May play a role in the assembly of respiratory supercomplexes (22342701). It is induced by hypoxia induction.This antibody is a rabbit polyclonal antibody raised against a full-length human HIGD1A protein.

{{ptg:RelatedPrimaryAntibodies}}