Anticorps Monoclonal anti-HIGD1A

HIGD1A Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3A8E2

N° de cat : 68231-1-PBS

Synonymes

RCF1 homolog A, Hypoxia-inducible gene 1 protein, HSPC010, HIG1 domain family member 1A, mitochondrial, HIG1 domain family member 1A

---

Galerie de données de validation

• 

  WB analysis using 68231-1-Ig (same clone as 68231-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68231-1-Ig (HIGD1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68231-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 68231-1-Ig (same clone as 68231-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using HIGD1A antibody (68231-1-Ig, Clone: 3A8E2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68231-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50454-2

  Cytometric bead array standard curve of MP50454-2, HIGD1A Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68231-1-PBS. Detection antibody: 68231-4-PBS. Standard:Ag14027. Range: 0.391-100 ng/mL.

---

Informations sur le produit

68231-1-PBS cible HIGD1A dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HIGD1A Protéine recombinante Ag14027
• Nom complet: HIG1 hypoxia inducible domain family, member 1A
• Masse moléculaire calculée: 93 aa, 10 kDa
• Poids moléculaire observé: 10 kDa
• Numéro d’acquisition GenBank: BC000601
• Symbole du gène: HIGD1A
• Identification du gène (NCBI): 25994
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HIG1 domain family member 1A (HIGD1A) is a proposed subunit of cytochrome c oxidase (COX, complex IV), which is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. May play a role in the assembly of respiratory supercomplexes (PMID:22342701). It is induced by hypoxia induction.

{{ptg:RelatedPrimaryAntibodies}}