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Anticorps Polyclonal de lapin anti-HIGD2A

HIGD2A Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21415-1-PBS

Synonymes

Galerie de données de validation

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  • Western Blot (WB) analysis of mouse kidney tissue using HIGD2A Polyclonal antibody (21415-1-AP)

    WB analysis of mouse kidney using 21415-1-AP (same clone as 21415-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21415-1-AP (HIGD2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21415-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using HIGD2A Polyclonal antibody (21415-1-AP)

    IF Staining of MCF-7 using 21415-1-AP (same clone as 21415-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HIGD2A antibody (21415-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21415-1-PBS in a different storage buffer formulation.

Informations sur le produit

21415-1-PBS cible HIGD2A dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène HIGD2A Protéine recombinante Ag15892
Nom complet HIG1 hypoxia inducible domain family, member 2A
Masse moléculaire calculée 106 aa, 12 kDa
Poids moléculaire observé11 kDa, 50 kDa
Numéro d’acquisition GenBankBC007502
Symbole du gène HIGD2A
Identification du gène (NCBI) 192286
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The mammalian HIGD-family proteins include two subgroups of yeast Rcf1 homologs, HIGD1A/B/C and HIGD2A/B. HIGD1A and HIGD2A (of 10.4 and 11.5 kDa, respectively) have two hydrophilic transmembrane domains and are localized in the inner mitochondrial membrane. HIGD1A and HIGD2A expression is induced under stress conditions like hypoxia or glucose deprivation in human cervical epithelial cells and murine cerebral cortical neurons. HIGD1A may play pleiotropic roles in mitochondrial respiratory chain biogenesis. HIGD2A forms a 50 kDa regulatory module with COX4-1, COX5B, and COX6A1, which binds to newly synthesized COX3 to promote its binding to the previously assembled COX1 + COX2 module (PMID:33291261,32375044).

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