Anticorps Polyclonal de lapin anti-HINFP

• Phare
• Validé par KD/KO

HINFP Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10066-2-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of human liver using 10066-2-AP (same clone as 10066-2-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 10066-2-AP (HINFP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10066-2-PBS in a different storage buffer formulation.

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  WB analysis using 10066-2-AP (same clone as 10066-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10066-2-AP (HINFP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10066-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 10066-2-AP (same clone as 10066-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10066-2-AP (HINFP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10066-2-PBS in a different storage buffer formulation.

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  IP experiment of L02 using 10066-2-AP (same clone as 10066-2-PBS)

  IP result of anti-HINFP (IP:10066-2-AP, 4ug; Detection:10066-2-AP 1:2000) with L02 cells lysate 1040 ug. This data was developed using the same antibody clone with 10066-2-PBS in a different storage buffer formulation.

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  IP experiment of L02 using 10066-2-AP (same clone as 10066-2-PBS)

  IP result of anti-HINFP (IP:10066-2-AP, 4ug; Detection:10066-2-AP 1:2000) with L02 cells lysate 1040 ug. This data was developed using the same antibody clone with 10066-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 10066-2-AP (same clone as 10066-2-PBS)

  Immunofluorescent analysis of fixed Hela cells using 10066-2-AP (HINFP antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 10066-2-PBS in a different storage buffer formulation.

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  IF Staining of L02 using 10066-2-AP (same clone as 10066-2-PBS)

  Immunofluorescent analysis of L02 cells using 10066-2-AP (HINFP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10066-2-PBS in a different storage buffer formulation.

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Informations sur le produit

10066-2-PBS cible HINFP dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HINFP Protéine recombinante Ag0115
• Nom complet: histone H4 transcription factor
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC001073
• Symbole du gène: HINFP
• Identification du gène (NCBI): 25988
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Methylation of CpG dinucleotides is the major epigenetic modification in higher eukaryotic genomes. MIZF interacts with MBD2, a methyl-CpG-binding protein playing a critical role in DNA methylation-mediated transcriptional repression. MIZF functions as a negative regulator of transcription by binding to MBD2 and recruiting histone deacetylase (HDAC)-containing complexes. MIZF mRNA is present in various human tissues and cell lines and expressed as a 60kd protein.

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