Anticorps Polyclonal de lapin anti-HIP1

• Phare
• Validé par KD/KO

HIP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 22231-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 22231-1-AP (same clone as 22231-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22231-1-AP (HIP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 22231-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 22231-1-AP (HIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 22231-1-AP (same clone as 22231-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 22231-1-AP (HIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 22231-1-AP (same clone as 22231-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 22231-1-AP (HIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 22231-1-AP (same clone as 22231-1-PBS)

  IP result of anti-HIP1 (IP:22231-1-AP, 4ug; Detection:22231-1-AP 1:1000) with HeLa cells lysate 2500ug. This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 22231-1-AP (same clone as 22231-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22231-1-AP (HIP1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 22231-1-AP (same clone as 22231-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22231-1-AP (HIP1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 22231-1-AP (same clone as 22231-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22231-1-AP (HIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 22231-1-AP (same clone as 22231-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22231-1-AP (HIP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 22231-1-AP (same clone as 22231-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22231-1-AP (HIP1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 22231-1-AP (same clone as 22231-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22231-1-AP (HIP1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 22231-1-AP (same clone as 22231-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22231-1-AP (HIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 22231-1-AP (same clone as 22231-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using HIP1 antibody (22231-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

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Informations sur le produit

22231-1-PBS cible HIP1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HIP1 Protéine recombinante Ag17576
• Nom complet: huntingtin interacting protein 1
• Masse moléculaire calculée: 1037 aa, 116 kDa
• Poids moléculaire observé: 116 kDa
• Numéro d’acquisition GenBank: BC110545
• Symbole du gène: HIP1
• Identification du gène (NCBI): 3092
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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