Anticorps Polyclonal de lapin anti-HIP14/ZDHHC17

HIP14/ZDHHC17 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30022-1-PBS

Synonymes

HIP14; ZDHHC17, ZDHHC17, Acyltransferase ZDHHC17, DHHC 17, DHHC domain-containing cysteine-rich protein 17

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Galerie de données de validation

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  WB analysis using 30022-1-AP (same clone as 30022-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30022-1-AP (HIP14; ZDHHC17 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30022-1-PBS in a different storage buffer formulation.

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  WB analysis using 30022-1-AP (same clone as 30022-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30022-1-AP (HIP14; ZDHHC17 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30022-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 30022-1-AP (same clone as 30022-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using HIP14; ZDHHC17 antibody (30022-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30022-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30022-1-PBS cible HIP14/ZDHHC17 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HIP14/ZDHHC17 Protéine recombinante Ag32654
• Nom complet: zinc finger, DHHC-type containing 17
• Masse moléculaire calculée: 73 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC050324
• Symbole du gène: ZDHHC17
• Identification du gène (NCBI): 23390
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ZDHHC17 is a neuronal palmitoyl transferase. Post-translational modification by the lipid palmitate is crucial for the correct targeting and function of many proteins. Polyglutamine expansions of huntingtin protein are responsible for the Huntington neurological disorder. ZDHHC17 gene encodes palmitoyl transferase huntingtin interacting protein (HIP14) which regulates palmitoylation and distribution of hungtingtin. The latter is implicated in the formation of inclusion bodies and enhanced neuronal toxicity. HIP14 was also shown to control neurotransmitter release.

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