Anticorps Polyclonal de lapin anti-HIP1R

• Phare
• Validé par KD/KO

HIP1R Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 16814-1-AP

Synonymes

HIP 12, HIP1 related protein, HIP12, HIP1R, HIP3, ILWEQ, KIAA0655

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Galerie de données de validation

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  WB analysis using 16814-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16814-1-AP (HIP1R antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 16814-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16814-1-AP (HIP1R Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16814-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16814-1-AP (HIP1R Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 16814-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16814-1-AP (HIP1R antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 16814-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16814-1-AP (HIP1R antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16814-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16814-1-AP (HIP1R antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 16814-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16814-1-AP (HIP1R antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 16814-1-AP

  IP Result of anti-HIP1R (IP:16814-1-AP, 4ug; Detection:16814-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human placenta using 16814-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16814-1-AP (HIP1R antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 16814-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16814-1-AP (HIP1R antibody) at dilution of 1:100 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HeLa, tissu cardiaque de souris, tissu hépatique humain, tissu pulmonaire de souris, tissu rénal de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

16814-1-AP cible HIP1R dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HIP1R Protéine recombinante Ag10353
• Nom complet: huntingtin interacting protein 1 related
• Masse moléculaire calculée: 615 aa, 70 kDa, 120 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC067085
• Symbole du gène: HIP1R
• Identification du gène (NCBI): 9026
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Cell; Targeting Pin1 renders pancreatic cancer eradicable by synergizing with immunochemotherapy.; Authors - Kazuhiro Koikawa ; KD Validated
human	WB	Nat Cell Biol; Acetylation-dependent regulation of PD-L1 nuclear translocation dictates the efficacy of anti-PD-1 immunotherapy.; Authors - Yang Gao ; KO Validated
human	WB	Nat Biomed Eng; Inhibiting PD-L1 palmitoylation enhances T-cell immune responses against tumours.; Authors - Han Yao
human	WB,IP,IF	Nat Chem Biol; HIP1R targets PD-L1 to lysosomal degradation to alter T cell-mediated cytotoxicity.; Authors - Huanbin Wang ; KD Validated
human	WB	Sci Adv; The Huntingtin-interacting protein SETD2/HYPB is an actin lysine methyltransferase.; Authors - Riyad N H Seervai ; KD Validated
human	IHC	J Clin Med; HIP1R Expression and Its Association with PD-1 Pathway Blockade Response in Refractory Advanced NonSmall Cell Lung Cancer: A Gene Set Enrichment Analysis.; Authors - Young Wha Koh