Anticorps Polyclonal de lapin anti-HIPK2

• Phare
• Validé par KD/KO

HIPK2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 55408-1-AP

Synonymes

PRO0593, Homeodomain-interacting protein kinase 2, hHIPk2, FLJ23711, EC:2.7.11.1

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Galerie de données de validation

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  WB analysis of mouse kidney using 55408-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55408-1-AP (HIPK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 55408-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55408-1-AP (HIPK2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 55408-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 55408-1-AP (HIPK2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 55408-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55408-1-AP (HIPK2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 55408-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 55408-1-AP (HIPK2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 55408-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 55408-1-AP (HIPK2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 55408-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55408-1-AP (HIPK2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 55408-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 55408-1-AP (HIPK2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 55408-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 55408-1-AP (HIPK2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris,
• Résultats positifs en IHC: tissu cérébral de souris, tissu cardiaque de rat, tissu cardiaque de souris, tissu cérébral de rat, tissu de carcinome à cellules rénales humain, tissu de gliome humain, tissu rénal de rat, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

55408-1-AP cible HIPK2 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: homeodomain interacting protein kinase 2
• Masse moléculaire calculée: 131 kDa
• Poids moléculaire observé: 131 kDa, 101 kDa
• Numéro d’acquisition GenBank: NM_022740
• Symbole du gène: HIPK2
• Identification du gène (NCBI): 28996
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HIPK2 is a serine/threonine kinase participating in transcriptional regulation, growth control, and apoptosis. The kinase contributes to the regulation of remarkably diverse pathways such as p53 activation or Wnt signaling. There is very low levels of HIPK2 protein expression in several thyroid tumors and the combination of HIPK2 and Gal-3,would be helpful in ameliorating the preoperative recognition of thyroid cancer. HIPK2 may represent a new tumour suppressor gene for these types of cancers and may constitute a new potential promising diagnostic marker and therapeutic target.(PMID:21698151).

Publications

Species	Application	Title
mouse	IF	EBioMedicine; Inhibition of HIPK2 protects stress-induced pathological cardiac remodeling; Authors - Qiulian Zhou
mouse	WB	Chem Biol Interact; HIPK2 overexpression relieves hypoxia/reoxygenation-induced apoptosis and oxidative damage of cardiomyocytes through enhancement of the Nrf2/ARE signaling pathway.; Authors - Xiaoyan Dang ; KD Validated
human	IHC	Microvasc Res; MiR-221-3p targets HIPK2 to promote diabetic wound healing.; Authors - Qiqi Yu ; KD Validated
human	WB,IHC	Cell Prolif; Homeodomain-interacting protein kinase 2 suppresses proliferation and aerobic glycolysis via ERK/cMyc axis in pancreatic cancer.; Authors - Yi Qin
human	WB	Int J Biol Macromol; Exosomal miR-1229 derived from colorectal cancer cells promotes angiogenesis by targeting HIPK2.; Authors - Hui-Ying Hu
human,mouse	CoIP,WB	Cell Res; Low glucose metabolite 3-phosphoglycerate switches PHGDH from serine synthesis to p53 activation to control cell fate; Authors - Yu-Qing Wu

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