Anticorps Recombinant de lapin anti-HIRA

HIRA Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240006F8

N° de cat : 83194-4-RR

Synonymes

240006F8, HIR, HIRA/HIR, Protein HIRA

---

Galerie de données de validation

• 

  WB analysis using 83194-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83194-4-RR (HIRA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of K-562 using 83194-4-RR

  1x10^6 K-562 cells were intracellularly stained with 0.25 ug HIRA Recombinant antibody (83194-4-RR, Clone:240006F8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  Affinity and Kinetic Characterization of 83194-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83194-4-RR against Human HIRA were performed. The affinity constant is below 1 pM.

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules K-562
• Résultats positifs en FC (Intra): cellules K-562,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83194-4-RR cible HIRA dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HIRA Protéine recombinante Ag34439
• Nom complet: HIR histone cell cycle regulation defective homolog A (S. cerevisiae)
• Masse moléculaire calculée: 112 kDa
• Poids moléculaire observé: 112 kDa
• Numéro d’acquisition GenBank: NM_003325
• Symbole du gène: HIRA
• Identification du gène (NCBI): 7290
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HIRA protein is encoded by a gene within a region of human chromosome 22q11.2 deleted in most patients with the DiGeorge syndrome, a developmental disorder, and was named for its amino acid sequence homology to two S. cerevisiae proteins, Hir1p and Hir2p. HIRA is critical in a specific chromatin assembly pathway that takes place independently of DNA synthesis (PMID: 12049744). HIRA complex associates with various proteins, such as transcription factors, RNA pol II, Prohibitin (PHB), and replication protein A (RPA) suggests that the targeting of the complex at specific locations may exploit additional structural properties and mechanisms (PMID: 30082790).

{{ptg:RelatedPrimaryAntibodies}}