Anticorps Recombinant de lapin anti-HIRA

HIRA Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240006E5

N° de cat : 83194-5-PBS

Synonymes

Protein HIRA, HIRA/HIR, HIR, 240006E5

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Galerie de données de validation

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  IF Staining of HeLa using 83194-5-RR (same clone as 83194-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIRA antibody (83194-5-RR, Clone: 240006E5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83194-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83194-5-RR (same clone as 83194-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIRA antibody (83194-5-RR, Clone: 240006E5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83194-5-PBS in a different storage buffer formulation.

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  FC experiment of K-562 using 83194-5-RR (same clone as 83194-5-PBS)

  1x10^6 K-562 cells were intracellularly stained with 0.25 ug HIRA Recombinant Antibody (83194-5-RR, Clone:240006E5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83194-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83194-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83194-5-RR against Human HIRA were performed. The affinity constant is 7.91 nM.

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Informations sur le produit

83194-5-PBS cible HIRA dans les applications de IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HIRA Protéine recombinante Ag34439
• Nom complet: HIR histone cell cycle regulation defective homolog A (S. cerevisiae)
• Masse moléculaire calculée: 112 kDa
• Numéro d’acquisition GenBank: NM_003325
• Symbole du gène: HIRA
• Identification du gène (NCBI): 7290
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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