Anticorps Recombinant de lapin anti-HIRA

HIRA Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240006E5

N° de cat : 83194-5-RR

Synonymes

Protein HIRA, HIRA/HIR, HIR, 240006E5

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Galerie de données de validation

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  IF Staining of HeLa using 83194-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIRA antibody (83194-5-RR, Clone: 240006E5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HeLa using 83194-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIRA antibody (83194-5-RR, Clone: 240006E5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of K-562 using 83194-5-RR

  1x10^6 K-562 cells were intracellularly stained with 0.25 ug HIRA Recombinant Antibody (83194-5-RR, Clone:240006E5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83194-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83194-5-RR against Human HIRA were performed. The affinity constant is 7.91 nM.

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Applications testées

• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules K-562,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83194-5-RR cible HIRA dans les applications de IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HIRA Protéine recombinante Ag34439
• Nom complet: HIR histone cell cycle regulation defective homolog A (S. cerevisiae)
• Masse moléculaire calculée: 112 kDa
• Numéro d’acquisition GenBank: NM_003325
• Symbole du gène: HIRA
• Identification du gène (NCBI): 7290
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HIRA protein is encoded by a gene within a region of human chromosome 22q11.2 deleted in most patients with the DiGeorge syndrome, a developmental disorder, and was named for its amino acid sequence homology to two S. cerevisiae proteins, Hir1p and Hir2p. HIRA is critical in a specific chromatin assembly pathway that takes place independently of DNA synthesis (PMID: 12049744). HIRA complex associates with various proteins, such as transcription factors, RNA pol II, Prohibitin (PHB), and replication protein A (RPA) suggests that the targeting of the complex at specific locations may exploit additional structural properties and mechanisms (PMID: 30082790).

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