Anticorps Polyclonal de lapin anti-Histone H1.5

Histone H1.5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 18093-1-PBS

Synonymes

HIST1H1B, histone cluster 1, H1b, Histone, H1F5, H1-5

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Galerie de données de validation

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  WB analysis using 18093-1-AP (same clone as 18093-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18093-1-AP (Histone H1.5 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18093-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 18093-1-AP (same clone as 18093-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 18093-1-AP (Histone H1.5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18093-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 18093-1-AP (same clone as 18093-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18093-1-AP (Histone H1.5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18093-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 18093-1-AP (same clone as 18093-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18093-1-AP (Histone H1.5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18093-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 18093-1-AP (same clone as 18093-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 18093-1-AP (Histone H1.5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18093-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 18093-1-AP (same clone as 18093-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 18093-1-AP (Histone H1.5 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18093-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 18093-1-AP (same clone as 18093-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Histone H1.5 antibody (18093-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18093-1-PBS in a different storage buffer formulation.

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Informations sur le produit

18093-1-PBS cible Histone H1.5 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Histone H1.5 Protéine recombinante Ag12818
• Nom complet: histone cluster 1, H1b
• Masse moléculaire calculée: 226 aa, 23 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC101581
• Symbole du gène: HIST1H1B
• Identification du gène (NCBI): 3009
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Histone H1.5, also known as Histone Cluster 1 H1 Family Member B (HIST1H1B), is a member of the Histone H1/H5 protein family. Histone H1.5 gene has been found to be frequently mutated in colorectal cancer and follicular lymphomas. HIST1H1B overexpression contributes to tumor aggressiveness. Histone H1.5 expression is upregulated in breast cancer and predicts poor prognosis(PMID: 34746019).

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