Anticorps Polyclonal de lapin anti-HIST1H1T

HIST1H1T Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

Avis (1)

N° de cat : 18188-1-AP

Synonymes

Histone H1t, Histone H1, Histone, H1t, H1FT

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Galerie de données de validation

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  WB analysis using 18188-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18188-1-AP (HIST1H1T antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 18188-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18188-1-AP (HIST1H1T antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 18188-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18188-1-AP (HIST1H1T antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 18188-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HIST1H1T antibody (18188-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu testiculaire humain,
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

18188-1-AP cible HIST1H1T dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HIST1H1T Protéine recombinante Ag12908
• Nom complet: histone cluster 1, H1t
• Masse moléculaire calculée: 206 aa, 22 kDa
• Poids moléculaire observé: 22-30 kDa
• Numéro d’acquisition GenBank: BC069517
• Symbole du gène: HIST1H1T
• Identification du gène (NCBI): 3010
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Testis-specific histone variants facilitate histone to protamine exchange during maturation of spermatozoa. Three testis-specific histone variants are regulated in male germ cells including H1T, H1T2 and HILS1 (encoded by H1ST1H1T, H1T2 and HILS1 respectively).

Publications

Species	Application	Title
mouse	WB	Cell Cycle; Function of RAD6B and RNF8 in spermatogenesis.; Authors - Yingli Guo
mouse	IF	Biol Reprod; UHRF1 is indispensable for meiotic sex chromosome inactivation and interacts with the DNA damage response pathway in mice.; Authors - Mengneng Xiong
mouse	IF	Adv Sci (Weinh); m6A Reader hnRNPA2B1 Modulates Late Pachytene Progression in Male Meiosis Through Post-Transcriptional Control; Authors - Lisha Yin

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ihsan (Verified Customer) (03-03-2023)

This antibody did not work in our hands to detect H1t or any other specific nuclear structure on mouse spermatocyte nuclear spreads, contrary to a previously published paper that used it and was able to detect H1t on same type of samples.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse spermatocyte nuclear spreads

HIST1H1T Antibody Immunofluorescence validation (1:100 dilution) in Mouse spermatocyte nuclear spreads (Cat no:18188-1-AP)

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