Anticorps Recombinant de lapin anti-Mono-Methyl-Histone H3 (Lys9)

Mono-Methyl-Histone H3 (Lys9) Recombinant Antibody for WB, Dot Blot, Indirect ELISA, ChIP-qPCR

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, Dot Blot, Indirect ELISA, ChIP-qPCR

Conjugaison

Non conjugué

CloneNo.

3C23

N° de cat : 82821-6-PBS

Synonymes

dmk9, H3 Me Lys9, H3 MeK9, H3 Methyl K9, H3 Methyl Lys9

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Galerie de données de validation

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  WB analysis using 82821-6-RR (same clone as 82821-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82821-6-RR (Mono-Methyl-Histone H3 (Lys9) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82821-6-PBS in a different storage buffer formulation.

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  Dot Blot experiment of peptide using 82821-6-RR (same clone as 82821-6-PBS)

  Dot blot analysis was used to confirm the specificity of 82821-6-RR Mono-Methyl-Histone H3 (Lys9) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 82821-6-PBS in a different storage buffer formulation.

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  ChIP experiment of HeLa using 82821-6-RR (same clone as 82821-6-PBS)

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Mono-Methyl-Histone H3 (Lys9) (82821-6-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 82821-6-PBS in a different storage buffer formulation.

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Informations sur le produit

82821-6-PBS cible Mono-Methyl-Histone H3 (Lys9) dans les applications de WB, Dot Blot, Indirect ELISA, ChIP-qPCR et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: histone cluster 1, H3a
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC066245
• Symbole du gène: HIST1H3A
• Identification du gène (NCBI): 8350
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified. Methylation of Histone H3 at lysine 9 is linked to transcriptional repression.

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