Anticorps Polyclonal de lapin anti-HJURP

• Phare
• Validé par KD/KO

HJURP Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(10)

Avis (1)

N° de cat : 15283-1-AP

Synonymes

FAKTS, FLEG1, hFLEG1, HJURP, Up regulated in lung cancer 9, URLC9

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Galerie de données de validation

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  WB analysis using 15283-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15283-1-AP (HJURP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 15283-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 15283-1-AP (HJURP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 15283-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 15283-1-AP (HJURP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HT-29, cellules A549, cellules HeLa, cellules MCF-7
• Résultats positifs en IHC: tissu cérébral humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 3 publications below

Informations sur le produit

15283-1-AP cible HJURP dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HJURP Protéine recombinante Ag7402
• Nom complet: Holliday junction recognition protein
• Masse moléculaire calculée: 86 kDa
• Poids moléculaire observé: 84 kDa
• Numéro d’acquisition GenBank: BC001940
• Symbole du gène: HJURP
• Identification du gène (NCBI): 55355
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Holliday Junction Recognition Protein (HJURP, also known as hFLEG1) is a centromeric protein with a pivotal role in the incorporation and maintenance of histone H3-like variant Centromere Protein-A (CENP-A) in centromeres. It is a specific chaperone for CENP-A and it integrates newly synthesized CENP-A molecules and nucleosomes at replicated centromeres. (PMID: 28819432, PMID: 31492860)

Publications

Species	Application	Title
human	WB	Dev Cell; CENP-A Ubiquitylation Is Indispensable to Cell Viability.; Authors - Yohei Niikura
human	IHC	Front Cell Dev Biol; Comprehensive Analysis Uncovers Prognostic and Immunogenic Characteristics of Cellular Senescence for Lung Adenocarcinoma.; Authors - Weihao Lin
human	IHC	Int J Cancer; Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma.; Authors - Fangqiu Fu
human	WB	J Biol Chem; Mitotic regulator Mis18β interacts with and specifies the centromeric assembly of molecular chaperone HJURP.; Authors - Jianyu Wang ; KD Validated
human	WB	Biochem Biophys Res Commun; Hypomethylation-driven overexpression of HJURP promotes progression of hepatocellular carcinoma and is associated with poor prognosis.; Authors - Ye Li ; KD Validated
		J Vis Exp; Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A).; Authors - Yohei Niikura

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christoffer (Verified Customer) (03-03-2025)

Adult mouse retina paraffin embedded and Cryo embedded embryonic (E14) mouse. The Cryo embedded tissue stains better with antigen retrieval (citric buffer).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: Embryonic (E14) mouse retina and adult mouse retina.

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