Anticorps Monoclonal anti-HJURP

HJURP Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F8C5

N° de cat : 68995-1-PBS

Synonymes

1F8C5, FAKTS, Fetal liver-expressing gene 1 protein, FLEG1, hFLEG1

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Galerie de données de validation

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  WB analysis using 68995-1-Ig (same clone as 68995-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68995-1-Ig (HJURP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68995-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 68995-1-Ig (same clone as 68995-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using HJURP antibody (68995-1-Ig, Clone: 1F8C5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 68995-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68995-1-PBS cible HJURP dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HJURP Protéine recombinante Ag34571
• Nom complet: Holliday junction recognition protein
• Masse moléculaire calculée: 86 kDa
• Poids moléculaire observé: 85-90 kDa
• Numéro d’acquisition GenBank: BC001940
• Symbole du gène: HJURP
• Identification du gène (NCBI): 55355
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Holliday Junction Recognition Protein (HJURP, also known as hFLEG1) is a centromeric protein with a pivotal role in the incorporation and maintenance of histone H3-like variant Centromere Protein-A (CENP-A) in centromeres. It is a specific chaperone for CENP-A and it integrates newly synthesized CENP-A molecules and nucleosomes at replicated centromeres. (PMID: 28819432, PMID: 31492860)

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