Anticorps Polyclonal de lapin anti-Hexokinase 1

• Phare
• Validé par KD/KO

Hexokinase 1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

31

Avis (2)

N° de cat : 19662-1-AP

Synonymes

Brain form hexokinase, Cell and organelle markers, Hexokinase 1, Hexokinase type I, HK I, HK1, HK1 ta, HK1 tb, HK1 tc, HKI, HXK1

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Galerie de données de validation

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  WB analysis using 19662-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19662-1-AP (Hexokinase 1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 19662-1-AP

  IP result of anti-Hexokinase 1(IP:19662-1-AP, 4ug; Detection:19662-1-AP 1:2000) with HeLa cells lysate 1720 ug.

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  IHC staining of mouse brain using 19662-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19662-1-AP (Hexokinase 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 19662-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19662-1-AP (Hexokinase 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules MCF-7, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 30 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

19662-1-AP cible Hexokinase 1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Hexokinase 1 Protéine recombinante Ag8015
• Nom complet: hexokinase 1
• Masse moléculaire calculée: 102 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC008730
• Symbole du gène: HK1
• Identification du gène (NCBI): 3098
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The hexokinases (HKs) catalyze the first obligatory step in glucose metabolism to generate glucose-6-phosphate. This not only furthers glucose entry by maintaining the concentration gradient for facilitated glucose influx but also provides the first intermediate for essentially all major pathways using glucose. There are four conventional HK isoforms, HK1/2/3/4, encoded by four different genes. Most adult tissues express only HK1. Muscle and adipose tissue use HK2 for glycolysis, liver, and pancreatic β cells express HK4 (also called glucokinase) and do not express HK1 or HK2. (PMID: 31434645, PMID: 31848318)

Publications

Species	Application	Title
mouse	WB	Nat Immunol; NF-κB-inducing kinase maintains T cell metabolic fitness in antitumor immunity.; Authors - Meidi Gu
human,mouse	WB,IP,IF	Sci Adv; Oligodendroglial glycolytic stress triggers inflammasome activation and neuropathology in Alzheimer's disease.; Authors - Xinwen Zhang
human	WB	Nat Commun; SUMOylation controls the binding of hexokinase 2 to mitochondria and protects against prostate cancer tumorigenesis.; Authors - Xun Shangguan
pig	WB	Br J Pharmacol; PMID: 30515768; Authors - Xiaoying Lian
human	WB	Cancer Lett; CYLD deficiency enhances metabolic reprogramming and tumor progression in nasopharyngeal carcinoma via PFKFB3.; Authors - Lingzhi Wang
mouse	WB	PLoS Biol; Loss of hepatic aldolase B activates Akt and promotes hepatocellular carcinogenesis by destabilizing the Aldob/Akt/PP2A protein complex.; Authors - Xuxiao He

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wei (Verified Customer) (01-30-2020)

Strong, clear band for mouse heart.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Mouse Heart

FH

SCOTT (Verified Customer) (11-20-2018)

Used 1 in/1000 in 5% BSA TBS-Tween overnight at 4 C rolling. The membrane was blocked in 5% BSA TBS-Tween prior to adding the antibody.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Human induced neuronal progenitor derived astrocytes

Hexokinase 1 Antibody Western Blot, validation (1/1000 dilution) in Human induced neuronal progenitor derived astrocytes (Cat no:19662-1-AP)