Anticorps Polyclonal de lapin anti-Hexokinase 2

• Phare
• Validé par KD/KO

Hexokinase 2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (5)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(329)

Avis (4)

N° de cat : 22029-1-AP

Synonymes

HK2, EC:2.7.1.1, Hexokinase type II, hexokinase2, Hexokinase-2

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Galerie de données de validation

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  WB analysis using 22029-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22029-1-AP (Hexokinase 2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 22029-1-AP

  IP result of anti-Hexokinase 2 (IP:22029-1-AP, 4ug; Detection:22029-1-AP 1:10000) with HeLa cells lysate 1520 ug.

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  IHC staining of human urothelial carcinoma using 22029-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 22029-1-AP (Hexokinase 2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 22029-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Hexokinase 2 antibody (22029-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules MCF-7, cellules RAW 264.7, tissu de muscle squelettique de rat, tissu de muscle squelettique de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de carcinome urothélial humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:20000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 12 publications below
• WB: See 300 publications below
• IHC: See 46 publications below
• IF: See 19 publications below
• IP: See 3 publications below
• CoIP: See 5 publications below
• RIP: See 1 publications below

Informations sur le produit

22029-1-AP cible Hexokinase 2 dans les applications de WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, canin, Humain, porc, poulet, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Hexokinase 2 Protéine recombinante Ag16895
• Nom complet: hexokinase 2
• Masse moléculaire calculée: 102 kDa
• Poids moléculaire observé: 102-120 kDa
• Numéro d’acquisition GenBank: BC021116
• Symbole du gène: Hexokinase 2
• Identification du gène (NCBI): 3099
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Hexokinase 2 (HK2), a rate-limiting enzyme in the first step of glycolysis pathway, expresses at high level in cancer cells compared with normal cells. HK2 provides a new target for cancer therapy due to its pivotal role in tumor tumourigenic and metastatic process. HK1 is constitutively expressed in most mammalian adult tissues. HK2, however, although is abundantly expressed in embryonic tissues, is expressed at high levels only in limited number of adult tissues such as adipose, skeletal, and cardiac muscles. (PMID: 29305912, PMID: 28427443, PMID: 23911236). The antibody also recognizes Hexokinase 1.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Acetate enables metabolic fitness and cognitive performance during sleep disruption; Authors - Qinqin He
mouse,human	WB	Cell Metab; An Upstream Open Reading Frame in Phosphatase and Tensin Homolog Encodes a Circuit Breaker of Lactate Metabolism.; Authors - Nunu Huang
mouse	WB	Cell Metab; STAT3 Activation-Induced Fatty Acid Oxidation in CD8+ T Effector Cells Is Critical for Obesity-Promoted Breast Tumor Growth.; Authors - Chunyan Zhang
	IP	Cell Metab; NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.; Authors - Mi Kyung Park
mouse	WB	Nat Neurosci; Metabolic lactate production coordinates vasculature development and progenitor behavior in the developing mouse neocortex.; Authors - Xiaoxiang Dong
human	WB	Nat Commun; Parvimonas micra promotes oral squamous cell carcinoma metastasis through TmpC-CKAP4 axis; Authors - Houbao Qi

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

SONAM (Verified Customer) (02-22-2024)

Works well

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: cancer cells, macrophages

FH

Maialen (Verified Customer) (10-03-2023)

It works very well

• Applications: Immunoprecipitation

Hexokinase 2 Antibody Immunoprecipitation validation ( dilution) in (Cat no:22029-1-AP)

FH

Macarena (Verified Customer) (10-07-2022)

excellent results. Very specific

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: cell lines

FH

Henar (Verified Customer) (08-31-2022)

Great antibody! No background and very specific result.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Primary mouse neurons

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