Anticorps Monoclonal anti-HK3

HK3 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

3H3C7

N° de cat : 67803-1-PBS

Synonymes

EC:2.7.1.1, Hexokinase 3, hexokinase 3 (white cell), Hexokinase type III, Hexokinase-3

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Galerie de données de validation

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  WB analysis of human saliva using 67803-1-Ig (same clone as 67803-1-PBS)

  human saliva were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

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  WB analysis of rat spleen using 67803-1-Ig (same clone as 67803-1-PBS)

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 67803-1-Ig (same clone as 67803-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

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  WB analysis of Ramos using 67803-1-Ig (same clone as 67803-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 67803-1-Ig (same clone as 67803-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67803-1-Ig (HK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67803-1-Ig (same clone as 67803-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67803-1-Ig (HK3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67803-1-Ig (same clone as 67803-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67803-1-Ig (HK3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67803-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67803-1-PBS cible HK3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HK3 Protéine recombinante Ag30798
• Nom complet: hexokinase 3 (white cell)
• Masse moléculaire calculée: 923 aa, 99 kDa
• Poids moléculaire observé: 99 kDa
• Numéro d’acquisition GenBank: BC028129
• Symbole du gène: HK3
• Identification du gène (NCBI): 3101
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Hexokinases phosphorylate glucose to produce glucose-6-phosphate (G6P), the first step in most glucose metabolism pathways. Hexokinase 3 also known as HK3, similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate.

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