Anticorps Polyclonal de lapin anti-HLA class I ABC

HLA class I ABC Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, RIP, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

35

Avis (2)

N° de cat : 15240-1-AP

Synonymes

HLA A, HLA class I, HLA class I (HLA-A), HLA class I ABC, HLA-A, MHC class I antigen A*3

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Galerie de données de validation

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  WB analysis using 15240-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15240-1-AP (HLA class I ABC antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 15240-1-AP

  IP Result of anti-HLA class I ABC (IP:15240-1-AP, 3ug; Detection:15240-1-AP 1:500) with HepG2 cells lysate 1720ug.

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  IHC staining of human tonsillitis using 15240-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15240-1-AP (HLA class I ABC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 15240-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15240-1-AP (HLA class I ABC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 15240-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15240-1-AP (HLA class I ABC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 15240-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15240-1-AP (HLA class I ABC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 15240-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using HLA class I ABC antibody (15240-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 15240-1-AP

  1X10^6 HepG2 cells were surface stained with 0.4 ug Anti-Human HLA class I ABC (15240-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were not fixed .

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HeLa, cellules HepG2
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer de l'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 18 publications below
• IHC: See 11 publications below
• IF: See 8 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

15240-1-AP cible HLA class I ABC dans les applications de WB, RIP, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HLA class I ABC Protéine recombinante Ag7370
• Nom complet: major histocompatibility complex, class I, A
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 44 kDa
• Numéro d’acquisition GenBank: BC003069
• Symbole du gène: HLA-A
• Identification du gène (NCBI): 3105
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)

Publications

Species	Application	Title
human	WB	Nature; Inhibition of PCSK9 potentiates immune checkpoint therapy for cancer.; Authors - Xinjian Liu
human	WB	Cell; Targeting Pin1 renders pancreatic cancer eradicable by synergizing with immunochemotherapy.; Authors - Kazuhiro Koikawa
human		Nat Cell Biol; An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles.; Authors - James W Clancy
	WB	ACS Appl Mater Interfaces; Self-Assembly of a Multifunction DNA Tetrahedron for Effective Delivery of Aptamer PL1 and Pcsk9 siRNA Potentiate Immune Checkpoint Therapy for Colorectal Cancer.; Authors - Wenjing Guo
human	WB	J Immunother Cancer; MYC suppresses STING-dependent innate immunity by transcriptionally upregulating DNMT1 in triple-negative breast cancer.; Authors - Si-Yu Wu
human	WB	J Immunother Cancer; THADA drives Golgi residency and upregulation of PD-L1 in cancer cells and provides promising target for immunotherapy.; Authors - Chushu Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Fabien (Verified Customer) (11-04-2022)

The antibody works great for western-blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: Jy cells

FH

Patricia (Verified Customer) (02-25-2020)

I tried probing my WB for 1 hour at RT, but it looked terrible, so I will try an overnight incubation. I've also tried my IP twice, once using 4 ug/mL, and the second time using 10 ug/mL. I had no pulldown either time. I'm quite disappointed.

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:1000 for WB; 4-10 ug/mL IP
• Cell Tissue Type: Ovarian cancer

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