Anticorps Polyclonal de lapin anti-HLA-A

HLA-A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 55383-1-AP

Synonymes

HLA A, HLA class I, HLA class I (HLA-A), HLA class I ABC, HLA class I histocompatibility antigen, A alpha chain

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Galerie de données de validation

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  WB analysis using 55383-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55383-1-AP (HLA-A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 55383-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55383-1-AP (HLA-A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 55383-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55383-1-AP (HLA-A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 55383-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 55383-1-AP (HLA-A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 55383-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 55383-1-AP (HLA-A Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 55383-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using HLA-A antibody (55383-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A431
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

55383-1-AP cible HLA-A dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: major histocompatibility complex, class I, A
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 41-44 kDa
• Numéro d’acquisition GenBank: NM_002116
• Symbole du gène: HLA-A
• Identification du gène (NCBI): 3105
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)

Publications

Species	Application	Title
human	IF	J Virol; Two Functional Variants of AP-1 Complexes Composed of either γ2 or γ1 Subunits Are Independently Required for Major Histocompatibility Complex Class I Downregulation by HIV-1 Nef.; Authors - Lucas A Tavares
human	IHC	Front Pharmacol; Proteomics study the potential targets for Rifampicin-resistant spinal tuberculosis; Authors - Yanling Wang

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