Anticorps Polyclonal de lapin anti-HLA-DMB

HLA-DMB Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

Avis (1)

N° de cat : 21704-1-AP

Synonymes

RING7, MHC class II antigen DMB, HLA DMB, DMB

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Galerie de données de validation

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  WB analysis using 21704-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21704-1-AP (HLA-DMB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 21704-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21704-1-AP (HLA-DMB antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 21704-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21704-1-AP (HLA-DMB antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Raji using 21704-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA-DMB antibody (21704-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Daudi, cellules Raji
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules Raji,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

21704-1-AP cible HLA-DMB dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HLA-DMB Protéine recombinante Ag16320
• Nom complet: major histocompatibility complex, class II, DM beta
• Masse moléculaire calculée: 263 aa, 29 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC027175
• Symbole du gène: HLA-DMB
• Identification du gène (NCBI): 3109
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). The class II molecules are composed of two non-covalently associated alpha and beta chains. HLA-DMA and HLA-DMB form a functional heterodimer that is critical in the pathway of class II antigen presentation. HLA-DM plays a critical role in catalyzing the release of Class II HLA-associated invariant chain-derived peptides (CLIP) from newly synthesized class II HLA molecules and freeing the peptide binding site for acquisition of antigenic peptides.

Publications

Species	Application	Title
human	WB	Nat Commun; Immunopeptidomics-based design of mRNA vaccine formulations against Listeria monocytogenes; Authors - Rupert L Mayer
human	WB,IF	Inflammation; RNA-seq Based Transcriptome Analysis Reveals Role of Myoglobin in Rheumatoid Arthritis; Authors - Haibin Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Adillah (Verified Customer) (04-20-2023)

good for western blot

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:1000

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