Anticorps Recombinant de lapin anti-HLA-DR

HLA-DR Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

L243

N° de cat : 65503-1-PBS

Synonymes

HLA-DRA, HLA DRA, HLA DRA1, HLA DRB, HLA-DRA1

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Galerie de données de validation

• 

  FC experiment of human PBMCs using 65503-1-RR (same clone as 65503-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human HLA-DR Rabbit Recombinant Antibody (65503-1-RR, Clone:L243) or Rabbit IgG control PolyAb and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed. Lymphocytes and monocytes were gated. This data was developed using the same antibody clone with 65503-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65503-1-PBS

  1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human HLA-DR Rabbit Recombinant Antibody (65503-1-PBS, Clone: L243) or Rabbit IgG control PolyAb and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed. Lymphocytes and monocytes were gated.

• 

  FC experiment of human PBMCs using 65503-1-PBS

  1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human HLA-DR Rabbit Recombinant Antibody (65503-1-PBS, Clone:L243) or Rabbit IgG control PolyAb and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed. Lymphocytes and monocytes were gated.

• 

  FC experiment of human PBMCs using 65503-1-RR (same clone as 65503-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human HLA-DR Rabbit Recombinant Antibody (65503-1-RR, Clone:L243) or Rabbit IgG control PolyAb and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed. Lymphocytes and monocytes were gated. This data was developed using the same antibody clone with 65503-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65503-1-RR (same clone as 65503-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human HLA-DR Rabbit Recombinant Antibody (65503-1-RR, Clone:L243) (left) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) or stained with 0.1 ug Anti-Human HLA-DR Mouse Monoclonal Antibody (Clone:L243) (center and right) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were not fixed. Lymphocytes and monocytes were gated. This data was developed using the same antibody clone with 65503-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65503-1-PBS

  1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human HLA-DR Rabbit Recombinant Antibody (65503-1-PBS, Clone:L243) (left) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) or stained with 0.1 ug Anti-Human HLA-DR Mouse Monoclonal Antibody (Clone:L243) (center and right) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were not fixed. Lymphocytes and monocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65503-1-PBS cible HLA-DR dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Human lymphoblastoid B-cell line RPMI 8866
• Nom complet: major histocompatibility complex, class II, DR alpha
• Masse moléculaire calculée: 254 aa, 29 kDa
• Numéro d’acquisition GenBank: BC032350
• Symbole du gène: HLA-DRA
• Identification du gène (NCBI): 3122
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. Stable for one year after shipment.

Informations générales

HLA-DR is a member of MHC class II moleculesencoded by the human leukocyte antigen complex on the region 6p21.31 of chromosome 6. HLA-DR is composed of two non-covalently associated transmembrane glycoproteins (the α and β chains), and is primarily expressed on B lymphocytes, monocytes, dendritic cells and thymic epithelial cells (PMID: 33125816). HLA-DR participate in the antigen presentation to CD4+ or CD8+ T cells and is postulated as a general biomarker of sepsis-induced immunosuppression (PMID: 28199206, 32421903). The Anti-Human HLA-DR antibody, clone L243, is derived from Human lymphoblastoid B-cell line RPMI 8866.

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