Anticorps Recombinant de lapin anti-HLA-DR

HLA-DR Recombinant Antibody for FC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

L243

N° de cat : 65560-1-MR

Synonymes

HLA-DRA, HLA DRA, HLA DRA1, HLA DRB, HLA-DRA1

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Galerie de données de validation

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  FC experiment of human PBMCs using 65560-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human HLA-DR Mouse IgG2a Recombinant Antibody (65560-1-MR, Clone: L243) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 65560-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human HLA-DR Mouse IgG2a Recombinant Antibody (65560-1-MR, Clone: L243)(red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65560-1-MR cible HLA-DR dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Human lymphoblastoid B-cell line RPMI 8866
• Nom complet: major histocompatibility complex, class II, DR alpha
• Masse moléculaire calculée: 254 aa, 29 kDa
• Numéro d’acquisition GenBank: BC032350
• Symbole du gène: HLA-DRA
• Identification du gène (NCBI): 3122
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

HLA-DR is a member of MHC class II molecules encoded by the human leukocyte antigen complex on the region 6p21.31 of chromosome 6. HLA-DR is composed of two non-covalently associated transmembrane glycoproteins (the α and β chains), and is primarily expressed on B lymphocytes, monocytes, dendritic cells and thymic epithelial cells (PMID: 33125816). HLA-DR participates in the antigen presentation to CD4+ or CD8+ T cells and is postulated as a general biomarker of sepsis-induced immunosuppression (PMID: 28199206, 32421903).

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