Anticorps Recombinant de lapin anti-HLA-DR

HLA-DR Recombinant Antibody for FC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

FC

Conjugaison

FITC Plus Fluorescent Dye

CloneNo.

L243

N° de cat : FITC-65560

Synonymes

HLA-DRA, HLA DRA, HLA DRA1, HLA DRB, HLA-DRA1

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Galerie de données de validation

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  FC experiment of human PBMCs using FITC-65560

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 647 Anti-Human CD14 and 5 ul FITC Plus Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant Antibody (FITC-65560, Clone: L243), or FITC Plus Mouse IgG2a Isotype Control (C1.18.4) (FITC-65208, Clone: C1.18.4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using FITC-65560

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant Antibody (FITC-65560, Clone: L243) (red), or FITC Plus Mouse IgG2a Isotype Control (C1.18.4) (FITC-65208, Clone: C1.18.4) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

FITC-65560 cible HLA-DR dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Human lymphoblastoid B-cell line RPMI 8866
• Nom complet: major histocompatibility complex, class II, DR alpha
• Masse moléculaire calculée: 254 aa, 29 kDa
• Numéro d’acquisition GenBank: BC032350
• Symbole du gène: HLA-DRA
• Identification du gène (NCBI): 3122
• Conjugaison: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

HLA-DR is a member of MHC class II molecules encoded by the human leukocyte antigen complex on the region 6p21.31 of chromosome 6. HLA-DR is composed of two non-covalently associated transmembrane glycoproteins (the α and β chains), and is primarily expressed on B lymphocytes, monocytes, dendritic cells and thymic epithelial cells (PMID: 33125816). HLA-DR participates in the antigen presentation to CD4+ or CD8+ T cells and is postulated as a general biomarker of sepsis-induced immunosuppression (PMID: 28199206, 32421903).

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