Anticorps Monoclonal anti-HLA-DRA

HLA-DRA Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3D6E8

N° de cat : 68543-1-PBS

Synonymes

HLA-DRA1, HLA-DR, HLA DRB, HLA DRA1, HLA DRA

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Galerie de données de validation

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  WB analysis using 68543-1-Ig (same clone as 68543-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68543-1-Ig (HLA-DRA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68543-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 68543-1-Ig (same clone as 68543-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68543-1-Ig (HLA-DRA antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68543-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 68543-1-Ig (same clone as 68543-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68543-1-Ig (HLA-DRA antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68543-1-PBS in a different storage buffer formulation.

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  IF Staining of Raji using 68543-1-Ig (same clone as 68543-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA-DRA antibody (68543-1-Ig, Clone: 3D6E8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68543-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68543-1-PBS cible HLA-DRA dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HLA-DRA Protéine recombinante Ag11224
• Nom complet: major histocompatibility complex, class II, DR alpha
• Masse moléculaire calculée: 254 aa, 29 kDa
• Poids moléculaire observé: 32-34 kDa
• Numéro d’acquisition GenBank: BC032350
• Symbole du gène: HLA-DRA
• Identification du gène (NCBI): 3122
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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