Anticorps Recombinant de lapin anti-HLA-DRB4

HLA-DRB4 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230352F10

N° de cat : 83103-1-PBS

Synonymes

MHC class II antigen DRB4, HLA DRB4, HLA DR4B, 230352F10

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Galerie de données de validation

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  WB analysis using 83103-1-RR (same clone as 83103-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83103-1-RR (HLA-DRB4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83103-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 83103-1-RR (same clone as 83103-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83103-1-RR (HLA-DRB4 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83103-1-PBS in a different storage buffer formulation.

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  IF Staining of Raji using 83103-1-RR (same clone as 83103-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA-DRB4 antibody (83103-1-RR, Clone: 230352F10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83103-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83103-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83103-1-RR against Human HLA-DRB4 were performed. The affinity constant is 0.623 nM.

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Informations sur le produit

83103-1-PBS cible HLA-DRB4 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HLA-DRB4 Protéine recombinante Ag8492
• Nom complet: major histocompatibility complex, class II, DR beta 4
• Masse moléculaire calculée: 266 aa, 30 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC005312
• Symbole du gène: HLA-DRB4
• Identification du gène (NCBI): 3126
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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