Anticorps Polyclonal de lapin anti-HLA-F

• Phare
• Validé par KD/KO

HLA-F Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 14670-1-PBS

Synonymes

HLA-5.4, HLA F antigen, HLA F, HLA CDA12, HLA 5.4

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Galerie de données de validation

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  WB analysis of Raji using 14670-1-AP (same clone as 14670-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 14670-1-AP (HLA-F antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14670-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 14670-1-AP (same clone as 14670-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 14670-1-AP (HLA-F antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14670-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 14670-1-AP (same clone as 14670-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14670-1-AP (HLA-F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14670-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 14670-1-AP (same clone as 14670-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 14670-1-AP (HLA-F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14670-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human spleen using 14670-1-AP (same clone as 14670-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 14670-1-AP (HLA-F antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 14670-1-AP (same clone as 14670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14670-1-AP (HLA-F antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 14670-1-AP (same clone as 14670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14670-1-AP (HLA-F antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 14670-1-AP (same clone as 14670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen slide using 14670-1-AP (HLA-F Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 14670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 14670-1-AP (same clone as 14670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen slide using 14670-1-AP (HLA-F Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 14670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 14670-1-AP (same clone as 14670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 14670-1-AP (HLA-F antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 14670-1-AP (same clone as 14670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 14670-1-AP (HLA-F antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 14670-1-AP (same clone as 14670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 14670-1-AP (HLA-F Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 14670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 14670-1-AP (same clone as 14670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 14670-1-AP (HLA-F Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 14670-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14670-1-PBS cible HLA-F dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HLA-F Protéine recombinante Ag6335
• Nom complet: major histocompatibility complex, class I, F
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: BC062991
• Symbole du gène: HLA-F
• Identification du gène (NCBI): 3134
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-F is a non-classical MHC class I molecule. (PMID: 667938; 3375250; 2249951)

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